Surface Electric Fields of Apatite Electret Promote Osteoblastic Responses
The osteoblast behaviors on the biomaterial substrates are recognized to play a fundamental role in osteoconduction process. The purpose of this study was to evaluate the in vitro behaviors of osteoblasts cultured on electrically polarized hydroxyapatite (HA), having the enhanced osteobonding abilities. Osteoblasts derived from mouse bone marrow were seeded onto the polarized HA and investigated the proliferation and differentiation. The polarization had effects on the proliferation of osteoblast precursor cells based on the MTT assay. The acceleration was emerged as the early achievement to the confluence on the N-HA and P-HA. The quantitative analysis of the results of ALP and AR-S staining, the charges induced on the HA surface accelerated the differentiation from the osteoblast precursor cells to mature osteoblasts.
Kunio Ishikawa and Yukihide Iwamoto
M. Nakamura et al., "Surface Electric Fields of Apatite Electret Promote Osteoblastic Responses", Key Engineering Materials, Vols. 529-530, pp. 357-360, 2013