Murine Macrophage RAW264.7 Cells Response for the Carbon Nanotubes Immobilized on Substrate
The reports on cytotoxic studies of carbon nanotubes (CNTs) increased exponentially. In the present study, we investigate murine macrophage RAW264.7 cell response for the CNTs immobilized on a polystyrene substrate. We prepared CNT-coated dishes, and estimate the interaction of RAW264.7 cells with CNTs by cell adhesion, proliferation assay, and measurement of TNF-α production. As a result, the highest cell adhesion and proliferation was observed on a commercially cell culture polystyrene dish, while CNT-coated dish indicate slightly lower activity of them. Moreover, amount of production of TNF-α on the CNT-coated dishes was considerable lower than that in the case of lipopolysaccharide (LPS) addition as a control. These results indicated that CNT-coated dishes could not show strong cytotoxicity for RAW264.7 cells in vitro.
Kunio Ishikawa and Yukihide Iwamoto
T. Akasaka et al., "Murine Macrophage RAW264.7 Cells Response for the Carbon Nanotubes Immobilized on Substrate", Key Engineering Materials, Vols. 529-530, pp. 379-384, 2013