In Vitro Evaluation of Silicon-Containing Apatite Fiber Scaffolds for Bone Tissue Engineering


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The aim of the present investigation was to examine Si release from the silicon-containing apatite fiber scaffold (Si-AFS) and the biocompatibility of the Si-AFS. We have successfully synthesized silicon-containing apatite fibers (Si-AF) by a homogenous precipitation method. Three-dimensional Si-AFS were fabricated using these Si-AFs. The concentrations of Si in the starting solution were 0 (AF) and 0.8 (0.8Si-AF) mass%. The 0.8Si-AFS1000 were fabricated by firing Si-AF slurry compacts (carbon/Si-AF [w/ ratio: 10/1) at 1300 °C for 5 h. Solubility experiments were carried out in 0.05 mol/dm3 Tris-HCl buffer solutions at pH 7.30 using 0.8Si-AFS1000 (porosity: ~98%), together with Si-free AFS1000 (~98%) for 21 days. The Ca2+, PO43- and SiO44- concentrations in the solution were determined by inductively-coupled plasma atomic emission spectrometry (ICP-AES). The biocompatibility of the Si-AFS was examined in vitro using osteoblastic cell, MC3T3-E1 for 21 days. The results of the ICP-AES analysis indicated that the amount of SiO44- ions released from 0.8Si-AFS1000 rapidly increased at 1 day, and then the released SiO44- ions remained constant over a period for 21 days. The cells seeded on/in the 0.8Si-AFS1000 well-proliferated as compared to those on/in the AFS1000. Consequently, we can conclude that the 0.8Si-AFS offers as a potential novel scaffold material, creating a three-dimensional cell culture environment.



Key Engineering Materials (Volumes 529-530)

Main Theme:

Edited by:

Kunio Ishikawa and Yukihide Iwamoto




Y. Kinoshita et al., "In Vitro Evaluation of Silicon-Containing Apatite Fiber Scaffolds for Bone Tissue Engineering", Key Engineering Materials, Vols. 529-530, pp. 391-396, 2013

Online since:

November 2012




[1] L. L. Hench, J. Am Ceram. Soc., 81, (1998) 1705-1728.

[2] M. Aizawa, H. Shinoda, H. Uchida, I. Okada, T. J. Fujimi, N. Kanzawa, H. Morisue, M. Matsumoto and Y. Toyama, In vitro biological evaluations of three-dimensional scaffold developed from single-crystal apatite fibres for tissue engineering of bone, Phosphorus Res. Bull., 17 (2004).


[3] M. Honda, T. Fujimi, S. Izumi, K. Izawa, M. Aizawa, H. Morisue, T. Tsuchiya, N. Kanzawa, Topographical analyses of proliferation and differentiation of osteoblasts in micro- and macropores of apatite-fiber scaffold, J. Biomed. Mater. Res.: Part A, 94A (2010).


[4] E.M. Carlisle, Silicon: A possible factor in bone calcification, Science, 167 (1970) 279-280.

[5] I. R. Gibson, S. M. Best and W. Bonfield, Chemical characterization of silicon-substituted hydroxyapatite, J. Biomed. Mater. Res., 44 (1999) 422-428.


[6] A. E. Porter, S. M. Best and W. Bonfield, J. Biomed. Mater. Res., 68A, pp.133-141 (2003).

[7] M. Aizawa, N. Patel, A. E. Poter, S. M. Best and W. Bonfield, Syntheses of Silicon-containing Apatite Fibres by a Homogeneous Precipitation Method and Their Characterization, Key Eng. Mater. 309-311 (2006) 1129-1132.


[8] Y. Kinoshita, S. M. Best, and M. Aizawa, Fabrication and evaluation of silicon-containing apatite fiber scaffolds for bone tissue engineering, Phosphorus Res. Bull., 26, 103-106 (2012).