Coprecipitation of DNA and Calcium Phosphate Using an Infusion Fluid Mixture

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A surface-mediated gene transfer system using DNA-calcium phosphate (CaP) composite layers (D-CaP layers) would be useful in tissue engineeing. In previous studies, D-CaP layers were fabricated in supersaturated CaP solutions prepared using chemical reagents. In this study, a so-called RKM solution prepared using clinically approved infusion fluids was employed as a supersaturated CaP solution. A D-CaP layer consisting of submicron spherical particles was successfully fabricated on a polystyrene substrate by immersing the substrate in the RKM solution for 24 h. When the immersion period was prolonged from 24 to 72 h, amount of CaP and DNA on the substrate increased. However, the gene transfer capability of the D-CaP layer for the CHO-K1 cells was kept unchanged irrespective of the immersion period. In the RKM solution process, immersion period of 24 h was found to be long enough for gene transfer application of the D-CaP layer. More importantly, the D-CaP layer fabricated by the RKM solution process exhibited a significantly higher gene transfer capability than our previous D-CaP layer fabricated in the conventional CaP solution with the same DNA concentration. The RKM solution process for the fabrication of D-CaP layers was found to be advantageous to the previous process in terms of not only safety but the layers gene transfer capability.

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Periodical:

Key Engineering Materials (Volumes 529-530)

Main Theme:

Edited by:

Kunio Ishikawa and Yukihide Iwamoto

Pages:

465-470

DOI:

10.4028/www.scientific.net/KEM.529-530.465

Citation:

A. Oyane et al., "Coprecipitation of DNA and Calcium Phosphate Using an Infusion Fluid Mixture", Key Engineering Materials, Vols. 529-530, pp. 465-470, 2013

Online since:

November 2012

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$35.00

[1] A. Oyane, X. Wang, Y. Sogo, A. Ito, H. Tsurushima, Calcium phosphate composite layers for surface-mediated gene transfer, Acta Biomater. 8 (2012) 2034-(2046).

DOI: 10.1016/j.actbio.2012.02.003

[2] H. Shen, J. Tan, W.M. Saltzman, Surface-mediated gene transfer from nanocomposites of controlled texture, Nature Mater. 3 (2004) 569-574.

DOI: 10.1038/nmat1179

[3] A. Oyane, H. Tsurushima, A. Ito, Highly efficient gene transfer system using laminin-DNA-apatite composite layer, J. Gene Med. 12 (2010) 194-206.

DOI: 10.1002/jgm.1425

[4] A. Oyane, M. Murayama, A. Yamazaki, Y. Sogo, A. Ito, H. Tsurushima, Fibronectin–DNA–apatite composite layer for highly efficient and area-specific gene transfer, J. Biomed. Mater. Res. A 92 (2010) 1038-1047.

DOI: 10.1002/jbm.a.32449

[5] A. Oyane, H. Tsurushima, Y. Yazaki, A. Hiroko, Y. Sogo, A. Ito A, A. Yamazaki, Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer, J. Mater. Sci. Mater. Med. 23 (2012) 1011-1019.

DOI: 10.1007/s10856-012-4581-y

[6] A. Oyane, H. Tsurushima, A. Ito, Novel gene-transferring scaffolds having a cell adhesion molecule–DNA–apatite nanocomposite surface, Gene Therapy 14 (2007) 1750-1753.

DOI: 10.1038/sj.gt.3303041

[7] B. Sun, K.K. Tran, H. Shen, Enabling customization of non-viral gene delivery systems for individual cell types by surface-induced mineralization, Biomater. 30 (2009) 6386-6393.

DOI: 10.1016/j.biomaterials.2009.08.006

[8] L.N. Luong, K.M. McFalls, D.H. Kohn, Gene delivery via DNA incorporation within a biomimetic apatite coating, Biomater. 30 (2009) 6996-7004.

DOI: 10.1016/j.biomaterials.2009.09.001

[9] Y. Yazaki, A. Oyane, Y. Sogo, A. Ito, A. Yamazaki, H. Tsurushima. Control of gene transfer on a DNA-fibronectin-apatite composite layer by the incorporation of carbonate and fluoride ions. Biomater. 32 (2011) 4896-4902.

DOI: 10.1016/j.biomaterials.2011.03.021

[10] W. Zhang, H. Tsurushima, A. Oyane, Y. Yazaki, Y. Sogo, A. Ito, A. Matsumura, BMP-2 gene–fibronectin–apatite composite layer enhances bone formation, J. Biomed. Sci. 18 (2011) 62.

DOI: 10.1186/1423-0127-18-62

[11] X. Wang, A. Oyane, H. Tsurushima, Y. Sogo, X. Li, A. Ito, BMP-2 and ALP gene expression induced by a BMP-2 gene-fibronectin-apatite composite layer, Biomed. Mater. 6 (2011) 045004.

DOI: 10.1088/1748-6041/6/4/045004

[12] M. Uchida, A. Oyane, H.M. Kim, T. Kokubo, A. Ito, Biomimetic coating of laminin–apatite composite on titanium metal with excellent cell adhesive property, Adv. Mater. 16 (2004) 1071-1074.

DOI: 10.1002/adma.200400152

[13] H. Mutsuzaki, A. Ito, Y. Sogo, M. Sakane, A. Oyane, N. Ochiai, Enhanced wound healing associated with Sharpey's fiber-like tissue formation around FGF-2-apatite composite layers on percutaneous titanium screws in rabbits, Arch. Orthop. Trauma Surg. 132 (2012).

DOI: 10.1007/s00402-011-1381-7

[14] A. Oyane, H. Tsurushima, A. Ito, Simple surface modification process to produce a transparent apatite–polystyrene composite for in situ observation of cell behavior, Chem. Lett. 35 (2006) 1300-1301.

DOI: 10.1246/cl.2006.1300

[15] K. Onuma, A. Ito, Cluster growth model for hydroxyapatite, Chem. Mater. 10 (1998) 3346-3351.

DOI: 10.1021/cm980062c

[16] A. Oyane, K. Onuma, T. Kokubo, A. Ito, Clustering of calcium phosphate in the system CaCl2-H3PO4-KCl-H2O, J. Phys. Chem. B 103 (1999) 8230-8235.

DOI: 10.1021/jp9910340

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