Characteristics of Surface Behavior and Osteoinductivity of Biomimetic Ceramic Scaffold


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We previously reported functionally graded hydroxyapatite (fg-HAp) with the characteristics of blood permeability into the bulk and osteoinduction by adding low dose of bone morphogenetic protein-2 (BMP-2). In this study, we evaluated the bioactivity of the obtained dense HAp bodies in a simulated body fluid (SBF) and the osteoinductive activity with or without SBF treatment in vivo. The fg-HApSBF was prepared by immersing the fg-HAp in the SBF solution at 14 days and the surface structure was observed by SEM. The fg-HApSBF showed fine bone-like crystal on the surface of the HAp. Ectopic bone formation occurred in the fg-HAp/BMP-2 (1.0, 0.5μg) system at 3 weeks, while only in the fg-HApSBF/BMP-2 (1.0μg), bone induction was found. The histological finding showed body fluid permeation into the fg-HApSBF bulk and bone formation were obserbed.



Key Engineering Materials (Volumes 529-530)

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Edited by:

Kunio Ishikawa and Yukihide Iwamoto




J. Tazaki et al., "Characteristics of Surface Behavior and Osteoinductivity of Biomimetic Ceramic Scaffold", Key Engineering Materials, Vols. 529-530, pp. 50-54, 2013

Online since:

November 2012




[1] L.L. Hench, Bioceramics: from concept to clinic, J. Am. Ceram. Soc. 72 (1993) 93-98.

[2] E.C. Shors, R.E. Holmes, Porous hydroxyapatite, In: L.L. Hench, J. Wilson, editors. An introduction to bioceramics. Singapore: World Scientific Publishing (1993) 181-198.


[3] J.C. Merry, I.R. Gibson, S.M. Best, W. Bonfield, Synthesis and characterization of carbonate hydroxyapatite, J. Mater. Sci. Mater. Med. 9 (1998) 779-783.

[4] D.H. Cormack, Ham's Histology. Philadelphia: Lippincott 6-8 (1987) 264-320.

[5] J. Tazaki, T. Akazawa, M. Murata, M. Yamamoto, Y. Tabata, R. Yoshimoto, M. Arisue, BMP-2 dose-response and release studies in functionally graded HAp, Key Engineering Materials 309-311 (2006) 965-968.


[6] M. Murata, T. Akazawa, J. Tazaki, K. Ito, T. Sasaki, M. Yamamoto, Y. Tabata, M. Arisue, Blood permeability of a novel ceramics scaffold for bone morphogenetic protein-2, J. Biomed. Mater. Res. Appl. Biomaterial 81B (2007) 469-475.


[7] T. Akazawa, M. Murata, T. Sasaki, J. Tazaki, M. Kobayashi, T. Kanno, K. Nakamura, M. Arisue, Biodegradation and bioabsorption innovation of the functionally graded bovine bone–originated apatite with blood permeability. J. Biomed. Mater. Res. 76A (2006).


[8] K. Hara, T. Kokubo, T. Nakamura, T. Yamamoto, Growth of a bonelike apatite layer on a substrate by a biomimetic process, J. Am. Ceram. Soc. 78 (1995) 1049-1053.

[9] D. Wangensteen, E.R. Weibel, Morphometric evaluation of chorioallantoic oxygen transport in the chick embryo, Respir. Physiol. 47 (1982) 1-20.


[10] E.R. Weibel, Stereological methods. practical methods for biological morphometry, New York: Academic Press; (1980).

[11] D.W. Martin, P.A. Mayes, V.W. Rodwell, Harper's Review of Biochemistry. Tokyo, Japan: Marzen, Lange; 1983: 561.