Time-Dependent Protein Adsorptive Behavior on/in Mesoporous Silica Materials

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The Time – Dependent Adsorptive Behavior of Biomolecules on/in Mesoporous Silica Materials (MPS) Have Been Studied by Using Transmission Electron Microscopy (TEM). Highly Ordered Hexagonal Mesoporous Silica Materials (SBA-15) Synthesized Were Immersed in Phosphate Buffer Saline (PBS) Solution Containing Cytochrome C for 2, 48, and 120 Hours at 4 °C. after that, MPS Materials Were Rinsed with PBS Solution, and then Immersed in PBS Solution without Cytochrome C. the Amount of Protein Adsorbed on/in MPS Materials Soaked for 2, 48, and 120 Hours Was 75 Mg/g, 135 Mg/g and 178 Mg/g, Respectively. the Time Profiles for Adsorption of Proteins Can Be Well Described by Intraparticle Diffusion Model. the Holes of MPS Materials Were Observed to Be Overlapped with Stained Proteins for First 2 Hours Immersion. the Stained Proteins Were Observed between Primary Particles and Partly inside the Mesoporous Channels in the MPS Material Immersed for 48 Hours. for MPS Immersed for 120 Hours, Stained Proteins Were Observed in Almost All Meso-Scale Channels of MPS. the Ratio of Desorbed Proteins from those Embedded on/in MPS Materials Was 13 % in Case of the MPS Materials Immersed in PBS Containing Cytochrome C for 2 Hours, while 8 % in Case of the MPS Materials Immersed for 48 and 120 Hours. Cytochrome C May Be Strongly Embedded on/in MPS Materials due to the Electrostatic Effect.

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Periodical:

Key Engineering Materials (Volumes 529-530)

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Edited by:

Kunio Ishikawa and Yukihide Iwamoto

Pages:

646-649

Citation:

Y. Yokogawa et al., "Time-Dependent Protein Adsorptive Behavior on/in Mesoporous Silica Materials", Key Engineering Materials, Vols. 529-530, pp. 646-649, 2013

Online since:

November 2012

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$38.00

[1] D. Zhao, Q. Huo, J. Feng, B.F. Chmelka, G.D. Stucky, J. Am. Chem. Soc., 120 (1998), pp.6024-6036.

[2] A. Katiyar, L. Ji, P. Smirniotis, N.G. Pinto, J. Chromatography A, Vol. 1069(2005), pp.119-126.

[3] Y. Kang, J. He, Z. Guo, Z. Guo, and Z. Song, Ind. Eng. Chem. Res., 46 (2007), pp.4474-4479.

[4] M. Miyahara, A. Vinu, K.Z. Hossain, T. Nakanishi, K. Ariga, Thin Solid Films, 499 (2006), pp.13-18.

[5] Q. Sun, L. Yang, Water Res., 37 (2003) pp.1535-1544.

[6] T.P.B. Nguyen, J-W Lee, W.G. Shim, and H. Moon, Microporous and Mesoporous Mater., 110 (2008), pp.560-69.

[7] Y. Yokogawa, A. Saito, N. Ogawa, A. Nakamura, I. Kishida, Key Eng. Mater., 493-494 (2012) pp.728-731.