Investigation of Multilayered Protein Adsorption on Carbonate Apatite with a QCM Technique
This study is concerned with multilayered protein adsorption on carbonate apatite (CAp) that is measured with a quartz crystal microbalance (QCM) technique. A carbonate apatite-deposited QCM sensor was prepared by electrophoretic deposition (EPD). On the CAp-deposited QCM sensor, fibrinogen adsorption followed by thrombin adsorption was investigated. The adsorption of fibrinogen on CAp led to a clear decrease in the resonance frequency of the sensor, which means that fibrinogen adsorbed on the CAp-deposited QCM sensor. The adsorbed amount of fibrinogen was larger on CAp than on a pristine sensor (Au). Although successive thrombin adsorption occurred on fibrinogen adlayers on both CAp and Au, the adsorbed amount and its conformation were different depending on the surface.
Kunio Ishikawa and Yukihide Iwamoto
T. Yoshioka et al., "Investigation of Multilayered Protein Adsorption on Carbonate Apatite with a QCM Technique", Key Engineering Materials, Vols. 529-530, pp. 74-77, 2013