Optimization of Fabrication Parameters to Prepare Tea Catechin-Loaded Liposomes by Fuzzy Orthogonality


Article Preview

The fuzzy orthogonal design is a method that the orthogonal results were fuzzed and then analyzed using fuzzy mathematical theory. It took the full advantage of the information from orthogonal experiments to obtain the optimal liposome preparation conditions by considering all the evaluation indexes. With the fuzzy comprehensive evaluation for the objective function, main technological parameters of process in liposome preparation were optimized using fuzzy orthogonal design. The optimal processing parameters were pH values of phosphate-buffer solution of 7,soybean phosphatidyl-choline and cholesterol proportion of 1:1,the concentration of Catechin of 5mg/mL,Oil­water proportion of 3:1.The catechin-loaded liposomes prepared under the optimized conditions has high encapsulation efficiency. The entrapment rate of catechin nanoliposome measured by validated test was 61.7%±0.015,while the mean diameter was 193.6±0.119 nm.



Key Engineering Materials (Volumes 531-532)

Edited by:

Chunliang Zhang and Liangchi Zhang




J. J. Fang et al., "Optimization of Fabrication Parameters to Prepare Tea Catechin-Loaded Liposomes by Fuzzy Orthogonality", Key Engineering Materials, Vols. 531-532, pp. 458-464, 2013

Online since:

December 2012




[1] Anand,P., Kunnumakara A.B., Sundaram,C., et al: Pharmaceutical Research Vol. 25(2008), pp.2097-2116.

[2] Middleton,E., Kandaswami,C. and Theoharides T.C.: Pharmacological Reviews Vol. 52(2000), pp.673-751.

[3] Cai,Y., Anavy N.D., and Chow H.H.S.: Drug Metabolism and Disposition Vol. 30(2002), pp.1246-1249.

[4] Dvorakova,K., Dorr R.T., Valcic,S. et al: Cancer Chemotherapy and Pharma-cology Vol. 43(1999), pp.331-335.

[5] Fang, J.Y., Lee, W.R., Shen, S.C. and Huang Y.L. et al: Journal of Dermatological Science Vol. 42(2006), pp.101-109.

[6] I. Peres,S. Rocha,J., Gomes,S. et al: Carbohydrate Polymers Vol. 86(2011), pp.147-153.

[7] A Shah, DT Hart . and Julia Powell. et al: Journal of Zool Vol. 44(2012), pp.23-29.

[8] Jain R.L., Shastri J.P.: Journal of Pharmaceutical Investigation Vol. 1(2011), pp.35-41.

[9] Hathout R.M., Mansour,S., Mortada N.D., et al: AAPS PharmSciTech Vol. 8(2007), p.1.

[10] Bhavane,R., Karathanasis,E., and Annapragada, et al: Int. J. Nanomed Vol. 407-418(2007), p.2.

[11] El-Samaligy, M.S., Afifi, N.N., Mahmoud, E.A., et al: Int. J. Pharm Vol. 319(2006), pp.121-129.

[12] Torchilin, V. P.: Drug Discov. vol. 4(2005), pp.145-160.