Evaluation of Surface Properties and In Vitro Characterization of Surface Modified In Situ TiO2 Nanofibers

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In situ TiO2 nanofiber arrays have been successfully produced directly on a Ti-6Al-4V substrate by using thermal oxidation under a limited supply of oxygen. Their morphology, elemental composition, crystal structure, surface roughness and surface wettability were characterized by field-emission scanning electron microscope (FESEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffractometer (XRD), atomic force microscope (AFM) and contact angle goniometer, respectively. The results of material characterization studies revealed that TiO2 nanofibers possessed greater surface roughness and wettability, as well as the degree of crystallinity. In vitro characterization have also been evaluated by using bovine articular chondrocytes on the resulting TiO2 nanofibrous surface at different time points. Cell adhesion was observed qualitatively by using FESEM and cell proliferation was determined quantitatively by using AlamarBlue reduction assay. The results showed that the TiO2 nanofibrous substrate triggers enhanced chondrocytes adhesion, proliferation, and production of extracellular matrix (ECM) fibrils compared to untreated substrate. These results suggest that the oxidation process produces a surface structure to which chondrocytes affinity, and thus this surface would has potential use in implants designed for cartilaginous applications.

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Periodical:

Key Engineering Materials (Volumes 656-657)

Edited by:

Jyh-Chen Chen, Usuki Hiroshi, Sheng-Wei Lee and Yiin-Kuen Fuh

Pages:

63-67

Citation:

A. W. Tan et al., "Evaluation of Surface Properties and In Vitro Characterization of Surface Modified In Situ TiO2 Nanofibers", Key Engineering Materials, Vols. 656-657, pp. 63-67, 2015

Online since:

July 2015

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$41.00

* - Corresponding Author

[1] W.Q. Yu, Y.L. Zhang, X.Q. Jiang, F.Q. Zhang, In vitro behavior of MC3T3-E1 preosteoblast with different annealing temperature titania nanotubes, Oral Dis 16 (7) (2010) 624-630.

DOI: https://doi.org/10.1111/j.1601-0825.2009.01643.x

[2] S. Ban, Y. Iwaya, H. Kono, H. Sato, Surface modification of titanium by etching in concentrated sulfuric acid, Dental Materials 22 (12) (2006) 1115-1120.

DOI: https://doi.org/10.1016/j.dental.2005.09.007

[3] X. Hu, H. Shen, K. Shuai, E. Zhang, Y. Bai, Y. Cheng, X. Xiong, S. Wang, J. Fang, S. Wei, Surface bioactivity modification of titanium by CO2 plasma treatment and induction of hydroxyapatite: In vitro and in vivo studies, Applied Surface Science 257 (6) (2011).

DOI: https://doi.org/10.1016/j.apsusc.2010.08.082

[4] A. Tavangar, B. Tan, K. Venkatakrishnan, Synthesis of bio-functionalized three-dimensional titania nanofibrous structures using femtosecond laser ablation, Acta Biomater 7 (6) (2011) 2726-2732.

DOI: https://doi.org/10.1016/j.actbio.2011.02.020

[5] A. Tan, B. Pingguan-Murphy, R. Ahmad, S. Akbar, Advances in fabrication of TiO2 nanofiber/nanowire arrays toward the cellular response in biomedical implantations: a review, J. Mater. Sci. 48 (24) (2013) 8337-8353.

DOI: https://doi.org/10.1007/s10853-013-7659-0

[6] A.W. Tan, B. Pingguan-Murphy, R. Ahmad, S.A. Akbar, Review of titania nanotubes: Fabrication and cellular response, Ceram. Int. 38 (6) (2012) 4421-4435.

DOI: https://doi.org/10.1016/j.ceramint.2012.03.002

[7] B. Dinan, D. Gallego-Perez, H, Lee, S.A. Akbar, Thermally grown TiO2 nanowires to improve cell growth and proliferation on titanium based materials, Ceram. Int. 39 (5) (2013) 5949-5954.

DOI: https://doi.org/10.1016/j.ceramint.2012.12.004

[8] A.W. Tan, A. Dalilottojari, B. Pingguan-Murphy, R. Ahmad, S. Akbar, In vitro chondrocyte interactions with TiO2 nanofibers grown on Ti-6Al-4V substrate by oxidation, Ceram. Int. 40 (6) (2014) 8301-8304.

DOI: https://doi.org/10.1016/j.ceramint.2014.01.032

[9] Y. Bai, I.S. Park, H.H. Park, M.H. Lee, T.S. Bae, W. Duncan, M. Swain, The effect of annealing temperatures on surface properties, hydroxyapatite growth and cell behaviors of TiO2 nanotubes, Surface and Interface Analysis 43 (6) (2011) 998-1005.

DOI: https://doi.org/10.1002/sia.3683