Effect of Hydroxyapatite Bioceramic Bodies on Subcutaneous Soft Tissue Reaction of Laboratory Rats

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Hydroxyapatite (HA) is widely used in bioceramic materials for bone grafting. HA scaffolds were synthesized using solid-state reaction method. Scaffolds were prepared by milling the elements of CaCO3 and NH4H2PO4 powders. The obtained powder was pressed with uniaxial pressing into a disc shape with the dimension of 4 mm in thickness and 16.5 mm in diameter under pressures 3 MPa and then sintering the samples at difference temperatures from 1100°C to 1300°C for 3 hours. This research aimed to produce phase HA scaffolds in order to find out the effects of sintering temperature on phase contents, density, porosity, hardness and bending strength, and to use optimized condition samples study with laboratory rats’ soft tissue to evaluate the soft tissue response to the samples. Thirty-two healthy in adults’ on non-gender-specific of Wistar rats were used in this study. Optimized, sintered samples were cut and lathed into a cylindrical shape. Sixty-four samples of optimized condition were implanted and left in subcutaneous tissue for 3, 7, 14, 21, 30, 45, 90 and 180 days. XRD, XRF, Archimedes technique, Vickers hardness and bending strength, as well as light microscopy, were used for analysis. The results of optimized condition have shown the bodies of sintered sample at 1300 °C for 3 hours had the highest content of 91.02 % HA phase, and the remaining phases of 4.51 % b-TCP and 4.47 % CaO, its bulk density and strength increased with increasing temperature, the highest bulk density of 2.006 ± 0.033 g/cm3, hardness of 30.02 ± 3.23 HV, bending strength of 9.07 ± 1.15 MPa. Sample reactions to soft tissues at 180 days were mild inflammatory cells, an absence of cellular infiltration, a presence of calcification, and absence of displacement of ceramic components into surrounding host tissue. Our results concluded that the samples were nontoxic to subcutaneous tissue and biocompatibility

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Dr. Somnuk Sirisoonthorn, Assist Prof. Dr. Sirithan Jiemsirilers, Dr. Siriphan Nilpairach, Assist Prof. Dr. Thanakorn Wasanapianpong, Assist Prof. Dr. Pornnapa Sujaridworakun, Assist Prof. Dr. Nutthita Chuankrerkkul

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12-17

Citation:

R. Koonawoot et al., "Effect of Hydroxyapatite Bioceramic Bodies on Subcutaneous Soft Tissue Reaction of Laboratory Rats", Key Engineering Materials, Vol. 690, pp. 12-17, 2016

Online since:

May 2016

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[1] K.S. Vecchio, X. Zhang, J.B. Massie, M. Wang, C.W. Kim, Conversion of Bulk Seashells to Biocompatible Hydroxyapatite for Bone implants, Acta Biomater. 3 (2007) 910-918.

DOI: https://doi.org/10.1016/j.actbio.2007.06.003

[2] B.D. Ratner., A.S. Hoffman., F.J. Schoen., J.E. Lemons, Biomaterials Science, first ed., Academic Press, San Diego, (1996).

[3] ASTM F1185-03, Standard Specification for Composition of Hydroxylapatite for Surgical Implants, West Conshohocken, PA, USA, 2009, pp.475-477.

[4] S. Raynaud, E. Champion, D. Bernahe-Assollant, P. Thomas, Calcium phosphate apatite with variable Ca/P atomic ratio I: Synthesis, characterization and thermal stability of powders, Biomaterials. 23 (2002) 1065-1072.

DOI: https://doi.org/10.1016/s0142-9612(01)00218-6

[5] L.M. Rodriquez-Lorenzo, M. Vallet-Regi, J.M.F. Ferreira, Fabrication of hydroxyapatite bodies by uniaxial pressing from a precipitated powder, Biomaterials. 22 (2001) 583-588.

DOI: https://doi.org/10.1016/s0142-9612(00)00218-0

[6] K.C.B. Yeong, J. Wang, S.C. Ng, Mechanochemical synthesis of nanocrystalline hydroxyapatite from CaO and CaHPO4, Biomaterials. 22 (2001) 2705-2712.

DOI: https://doi.org/10.1016/s0142-9612(00)00257-x

[7] S. Pramanik, A.K. Agarwal, K.N. Rai, A. Garg, Development of high strength hydroxyapatite by solid-state-sintering process, Ceram. Int. 33 (2007) 419-426.

DOI: https://doi.org/10.1016/j.ceramint.2005.10.025

[8] S. Callut, J.C. Knowles, Correlation between structure and compression strength in reticulated glass-reinforced hydroxyapatite foam, J. Mater. Sci. Mater. Med. 13 (2000) 485-489.

[9] G. Dewith, H.H.M. Wagemans, Ball-on-ring test revisited, J. Am. Ceram Soc. 72 (1989) 1538-1541.

[10] W.D. Callister, Fundamentals of Material Science and Engineering, Willey, New York, (2001).

[11] Committee for the Update of the Guide for the Care and Use of Laboratory Animals, Guide for the Care and Use of Laboratory Animals, eighth ed., The National Academies Press, Washington, DC, (2011).

DOI: https://doi.org/10.17226/12910

[12] ASTM F981-04, Standard Practice for Assessment of Compatibility of Biomaterials for Surgical Implants with Respect to Effect of Materials on Muscle and Bone, West Conshohocken, PA, USA, 2010, pp.405-409.

[13] ASTM F763-04, Standard Practice for Short-Term Screening of Implant Materials, West Conshohocken, PA, USA, 1997, pp.694-697.

[14] Y.M. How, S.R. Kasim, H.M. Akil and Z.A. Ahmad, Effect of CaCO3 Particle Size in Synthesis of b-TCP Powder, JNRT. 6(1) (2009) 19-24.

[15] A. Farzadi, M. Solati-Hashjin, F. Bakhshi and A. Aminian, Synthesis and characterization of hydroxyapatite/b-tricalcium phosphate nanocomposite using microwave irradiation, Ceram. Int. 37 (2011) 65-71.

DOI: https://doi.org/10.1016/j.ceramint.2010.08.021

[16] M.N. Rahaman, Ceramic Processing and Sintering, second ed., Marcel Dekker, New York, (2003).

[17] M. Wang, Bioactive materials and processing, in: D. Shi (Eds. ), Biomaterials and tissue engineering, Heidelberg., Springer Verlag, Berlin, 2004, pp.1-82.