Effect of Silicate Incorporation in Alpha-Tricalcium Phosphate on Behaviors of Osteoblast-Like Cells

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Silicate-containing alpha-tricalcium phosphate (α-TCP) ceramics are expected to be useful scaffolds for bone regeneration because α-TCP shows high biodegradability and silicate ions are expected to promote the bone formation. We previously revealed that the porous silicate-containing α-TCP granules provided earlier bone formation and showed lower biodegradability than the porous silicate-free α-TCP granules in vivo. In order to reveal the mechanism of the bone formation promoted by silicate incorporation, the proliferation and differentiation of osteoblast-like cells on the silicate-containing and silicate-free α-TCP ceramics were examined in vitro. The silicate incorporation in α-TCP promoted the differentiation of osteoblast-like cells, and it might be one of the factors to promote bone formation In Vivo.

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Edited by:

Ahmed El-Ghannam

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90-94

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M. Kamitakahara et al., "Effect of Silicate Incorporation in Alpha-Tricalcium Phosphate on Behaviors of Osteoblast-Like Cells", Key Engineering Materials, Vol. 720, pp. 90-94, 2017

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November 2016

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[1] R.Z. LeGeros, Properties of osteoconductive biomaterials: calcium phosphates, Clin. Orthop. Relat. Res. 395 (2002) 81–98.

[2] M. Yamada, M. Shiota, Y. Yamashita, S. Kasugai, Histological and histomorphometrical comparative study of the degradation and osteoconductive characteristics of a- and b-tricalcium phosphate in block grafts, J. Biomed. Mater. Res. B: Appl. Biomater. 82B (2007).

DOI: https://doi.org/10.1002/jbm.b.30715

[3] R.G. Carrodeguas, S. De Aza, α-Tricalcium phosphate: synthesis, properties and biomedical applications. Acta Biomater. 7 (2011) 3536–46.

DOI: https://doi.org/10.1016/j.actbio.2011.06.019

[4] N. Patel, S.M. Best, W. Bonfield, I.R. Gibson, K.A. Hing, E. Damien, P.A. Revell, A comparative study on the in vivo behavior of hydroxyapatite and silicon substituted hydroxyapatite granules, J. Mater. Sci. Mater. Med. 13 (2002) 1199–206.

[5] I. Massie, J.M.S. Skakle, I.R. Gibson, Synthesis and phase stability of silicate-substituted α-tricalcium phosphate, Key Eng. Mater. 361–363 (2008) 67–70.

DOI: https://doi.org/10.4028/www.scientific.net/kem.361-363.67

[6] J. Duncan, S. Hayakawa, A. Osaka, J.F. MacDonald, J.V. Hanna, J.M.S. Skakle, I.R. Gibson, Furthering the understanding of silicate-substitution in α-tricalcium phosphate: an X-ray diffraction, X-ray fluorescence and solid-state nuclear magnetic resonance study, Acta Biomater. 10 (2014).

DOI: https://doi.org/10.1016/j.actbio.2013.11.014

[7] M. Kamitakahara, T. Kurauchi, M. Tanihara, K. Ioku, C. Ohtsuki, Synthesis of Si-containing tricalcium phosphate and its sintering behavior, Key Eng. Mater. 361–363 (2008) 59–62.

DOI: https://doi.org/10.4028/www.scientific.net/kem.361-363.59

[8] M. Kamitakahara, E. Tatsukawa, Y. Shibata, S. Umemoto, T. Yokoi, K. Ioku, T. Ikeda, Effect of silicate incorporation on in vivo responses of alpha-tricalcium phosphate ceramics, J. Mater. Sci. Mater. Med. 27 (2016) 97 (9pp).

DOI: https://doi.org/10.1007/s10856-016-5706-5

[9] M. Kamitakahara, S. Umemoto, K. Ioku, Characterization and in vitro evaluation of silicate-containing tricalcium phosphate prepared through wet chemical process. Key Eng. Mater. 529–530 (2013) 105–8.

DOI: https://doi.org/10.4028/www.scientific.net/kem.529-530.105

[10] J.W. Reid, L. Tuck, M. Sayer, K. Fargo, J.A. Hendry, Synthesis and characterization of single-phase silicon-substituted α-tricalcium phosphate, Biomaterials, 27 (2006) 2916–25.

DOI: https://doi.org/10.1016/j.biomaterials.2006.01.007

[11] L. Fei, C. Wang, Y. Xue, K. Lin, J. Chang, J. Sun, Osteogenic differentiation of osteoblasts induced by calcium silicate and calcium silicate/b-tricalcium phosphate composite bioceramics, J. Biomed. Mater. Res. Part B 100B (2012) 1237–44.

DOI: https://doi.org/10.1002/jbm.b.32688