The Study of Apoptosis and Phagocytosis of Rabbit Synoviocytes Induced by Particulate Joint Replacement Materials

Abstract:

Article Preview

Synoviocyte is one of the cells in direct contact with the wear particles and the role of synoviocytes in the prosthesis loosening remains to be further understood. The purpose of the present study is to investigate whether particulate biomaterials cause synoviocyte dead or simply activate these cells. The activation of synoviocyte in response to particulate biomaterial exposure will be indicated with its phagocytotic ability. As to the effects of particulate biomaterials to apoptosis will be tested on rabbit synoviocytes, HIG-82 cells. The results demonstrate that the addition of cobalt particles would lead the HIG-82 cells to apoptosis and apoptosis accompanying caspase3 cleavage, which means that apoptosis of the HIG-82 cells resulted from the addition of cobalt particles might went through caspase-dependent pathway. On the other hand, exposing to nonmetal particles such as TiO2 and SiO2, particles did not provoke apoptosis of the HIG-82 cells. TiO2 particles activated the cells and were phagocytosed by the HIG-82 synoviocytes, no significant biological effects were found while the HIG-82 cells exposed to SiO2 particles.

Info:

Periodical:

Materials Science Forum (Volumes 475-479)

Main Theme:

Edited by:

Z.Y. Zhong, H. Saka, T.H. Kim, E.A. Holm, Y.F. Han and X.S. Xie

Pages:

2419-2422

Citation:

C. T. Yung et al., "The Study of Apoptosis and Phagocytosis of Rabbit Synoviocytes Induced by Particulate Joint Replacement Materials", Materials Science Forum, Vols. 475-479, pp. 2419-2422, 2005

Online since:

January 2005

Export:

Price:

$38.00

[1] Rae T. J: Bone Joint Surg Br. 57(1975) 444-450.

[2] Evans E.J.: Biomaterials 15, 713-717. (1994).

[3] Mosmann T. J: Immunol Methods Dec 16; 65(1-2) (1983) pp.55-63..

[4] Jeppesen C. and Nielsen P.E.: Eur. J Biochem 182(1989) pp.437-444.

[5] Andree H.A., Reutelingsperger, C.P., Hauptmann R., Hemker H.C., Hermens W.T., and Willems G.M.: J Biol Chem 265(1990) pp.4923-4928.

[6] Chang H.H., Lin C.H., and Lo, S. J.: Exp Cell Res 250(1999) pp.387-400.

[7] Mahdi F., Madar Z.S., Figueroa C.D., and Schmaier A.H.: Blood 99 (2002) pp.3585-3596.

Fetching data from Crossref.
This may take some time to load.