Head-On Immobilization of DNA Fragments on CVD-Diamond Layers

Abstract:

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Synthetic diamond is regarded as a promising material for biosensors: it forms a stable platform for genetic assays and its biocompatibility opens the possibility for in vivo sensing. In this study the use of a thymidine linker for covalent DNA attachment was evaluated. Contact angle measurements provided a qualitative test of the initially oxidized surface. X-ray photoemission spectroscopy was used for further analysis of the oxides and for monitoring the effect of subsequent chemical treatments. The presence of FITC-labelled DNA was confirmed by confocal fluorescence microscopy. Enzyme linked immunosorbent assays indicated that this DNA was merely adsorbed on the diamond surface instead of covalently bound.

Info:

Periodical:

Materials Science Forum (Volumes 492-493)

Edited by:

Omer Van der Biest, Michael Gasik, Jozef Vleugels

Pages:

267-272

DOI:

10.4028/www.scientific.net/MSF.492-493.267

Citation:

S. Wenmackers et al., "Head-On Immobilization of DNA Fragments on CVD-Diamond Layers", Materials Science Forum, Vols. 492-493, pp. 267-272, 2005

Online since:

August 2005

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Price:

$35.00

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