Head-On Immobilization of DNA Fragments on CVD-Diamond Layers
Synthetic diamond is regarded as a promising material for biosensors: it forms a stable platform for genetic assays and its biocompatibility opens the possibility for in vivo sensing. In this study the use of a thymidine linker for covalent DNA attachment was evaluated. Contact angle measurements provided a qualitative test of the initially oxidized surface. X-ray photoemission spectroscopy was used for further analysis of the oxides and for monitoring the effect of subsequent chemical treatments. The presence of FITC-labelled DNA was confirmed by confocal fluorescence microscopy. Enzyme linked immunosorbent assays indicated that this DNA was merely adsorbed on the diamond surface instead of covalently bound.
Omer Van der Biest, Michael Gasik, Jozef Vleugels
S. Wenmackers et al., "Head-On Immobilization of DNA Fragments on CVD-Diamond Layers", Materials Science Forum, Vols. 492-493, pp. 267-272, 2005