Nano-hydroxyapatite (n-HA) slurry was synthesized at normal pressure, and (Cu2+, Zn2+)-bearing nano-hydroxyapatite (Cu-Zn-HA) was prepared by ion exchange reaction in water medium. The properties of n-HA and Cu-Zn-HA were characterized by AAS, TEM, XRD and FTIR. The results of XRD analysis indicate that Cu2+ and Zn2+ can occupy Ca2+ sites and enter the crystal lattice of hydroxyapatite. Through the antibacterial experiments, it was found that Cu-Zn-HA had better antibacterial ability on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). After heat treatment at 400°C for an hour, the materials still had good antibacterial effect. The accelerated aging method was employed to test antibacterial durability of the material and the results indicated that the durability was good. The results of the security assessment, including skin stimulation test of rabbit, acute toxicity test by stomach filling and the micronucleus test in bone marrow polychromatic erythrocytes of mice, showed that the security of the material was excellent.