Segmented polyurethanes (PUR) based on poly(ε-caprolactone)diol (PCL) as soft segments and cycloaliphatic diisocyanate were obtained. Two different types of catalysts were used for synthesis: dibutyltin dilaurate (DBTDL) and iron (III) acetylacetonate Fe(acac)3. Structure, thermal analysis, water absorption of obtained polyurethanes were studied. Polyurethanes catalyzed by Fe(acac)3 have lower glass transition temperature (Tg2) and melting temperature (Tm2) of hard domains in comparison with polyurethanes synthesized with DBTDL. Amount of absorbed water is in the range of 1,0-2,0% and is higher for PUR catalyzed by DBTDL. SEM observations show that type of catalyst used in synthesis affect structure of polyurethanes. Biological test XTT was done in order to determine influence of various metallic compounds used as catalyst on viability of human bone cell culture. The XTT results indicate no influence on viability of human osteoblasts cultured on the obtained PUR.