Immobilization of Thrombin Inhibitors on Polyesters Surface: An Original Approach towards Materials Blood Compatibilization
Piperazinyl-amide derivatives of N--(3-trifluoromethyl-benzenesulfonyl)-L-arginine were synthesized as graftable thrombin inhibitors. Their biological activity was evaluated in vitro, against human -thrombin, and in blood coagulation assay. The piperazinyl-amide derivatives were found to inhibit the activity of -thrombin in the micromolar range. The designed molecules were fixed on poly(ethylene terephthalate) (PET), and poly(butylene terephthalate) (PBT) by wet chemistry treatment (activation of hydroxyl chain-ends) and photochemistry (nitrene insertion by photoactivation of aromatic azide). The protocols were validated by X-ray photoelectron spectroscopy (XPS) and by radiochemical assay (liquid scintillation counting, LSC).
Paula Maria Vilarinho
C. Salvagnini and J. Marchand-Brynaert, "Immobilization of Thrombin Inhibitors on Polyesters Surface: An Original Approach towards Materials Blood Compatibilization", Materials Science Forum, Vols. 514-516, pp. 961-965, 2006