Enzymatic Degradation Behaviour of Starch Conjugated Phosphorylated Chitosan


Article Preview

Phosphorylated chitosan (P-chitosan) was synthesized by means of graft copolymerization technique. The conjugate membranes were prepared from oxidised starch and Pchitosan using reductive alkylation crosslinking. The synthesized membranes were characterised by FT-IR. In order to characterize degradation behaviour of this conjugated system, the membranes were incubated in enzyme solutions of alpha-amylase and lysozyme as well as a physiological saline solution (PBS) used as control solution. In PBS, low starch containing membranes (0.16-0.38 weight (starch)/weight (P-chitosan), (ws/wc)) and control membranes have not showed significant change in their weight during two months of incubation. High starch containing membranes (0.73-1.04 ws/wc) indicated less than 20 % weight loss after this period. After α-amylase incubation, a distinct degradation behaviour was observed from starch-P-chitosan membranes. The degradation of the conjugate membranes was found to be fast with increasing starch content. Weight losses between 20 to 55 % were detected for the lowest (0.16 ws/wc) starch and highest (1.04 ws/wc) starch containing membranes, respectively. In the lysozyme degradation study, the conjugate membranes were not degraded by enzymatic activity and the weights of membranes were seen to be increased about 20 % because of swelling. The control membranes showed gradual weight loss in enzyme solutions. These results indicated the lysozyme degradation of starch-free P-chitosan membranes and inhibition of degradation P-chitosan by highly conjugated starch molecules.



Materials Science Forum (Volumes 514-516)

Edited by:

Paula Maria Vilarinho




E. T. Baran et al., "Enzymatic Degradation Behaviour of Starch Conjugated Phosphorylated Chitosan", Materials Science Forum, Vols. 514-516, pp. 995-999, 2006

Online since:

May 2006




[1] R. Kennedy, D.J. Costain, V.C. McAlister, T.D.G. Lee: Surgery Vol. 120 (1996), p.866.

[2] M. Gingras, I. Paradis, and F. Berthod: Biomaterials Vol. 24 (2003), p.1653.

[3] M.M. Amiji: J. Bioma. Sci. Polymer Edn. Vol. 8 (1996), p.281.

[4] R.A.A. Muzzarelli: Carbohyd. Polym. Vol. 20 (1993), p.7.

[5] S. Chen and Y. Wang: J. Appl. Polym. Sci. Vol. 82 (2001), p.2414.

[6] Z.K. Yang and Y. Yuan: J. Appl. Polym. Sci. Vol. 82 (2001), p.1838.

[7] S. Chen and Y. Wang: J. Appl. Polym. Sci. Vol. 82 (2001), p.2414.

[8] B.O. Jung, C.H. Kim, K.S. Choi, Y.M. Lee and J.J. Kim: J. Appl. Polym. Sci. Vol. 72 (1999), p.1713.

[9] A.F. Kotze, H.L. Luessen, B.J. De Leeuw, A.G. De Boer, J.C. Verhoef and H.E. Junginger: Pharm. Res. Vol. 14 (1997), p.1197.

[10] M. Thanou, J.C. Verhoef and H.E. Junginger: Adv. Drug. Del. Rev. Vol. 52 (2001), p.117.

[11] A.S. Hoffman, G. Chen, X. Wu, Z. Ding, B. Kabra, K. Randeri, M. Schiller, E. Ron, N.A. Peppas and C. Brazil: Polym. Prep. Vol. 38 (1997), p.524.

[12] R.A. Tasker, B.J. Connell, S.J. Ross and C.M. Elson: Lab Ani. Vol. 32 (1998), p.270.

[13] K. Ono, Y. Saito, H. Yura and K. Ishikawa: J. Biomed. Mater. Res. Vol. 49 (2000), p.289.

[14] D.K. Singh and A.R. Ray: Carbohydr. Polym. Vol. 36 (1998), p.251.

[15] Y.M. Lee and E.M. Shin: J. Mem. Sci. Vol. 64, (1991) p.145.

[16] N. Nishi, A. Ebina, S. Nishimura, A. Tsutsumi, O. Hasegawa and S. Tokura: Int. J. Biol. Macromol. Vol. 8 (1986), p.311.

[17] E.T. Baran, J. F Mano, R.L. Reis: J. Mat. Sci: Mater. Med. Vol. 15 (2004), p.759.