A Prototype Continuous Flow Polymerase Chain Reaction LTCC Device


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There is a growing need for remote biological sensing in both laboratory and harsh field environments. Sensing and detection of biological entities such as anthrax, Ebola and other micro-organisms of interest involves sampling of the environment, amplification, analysis and identification of the target DNA. A key component of such a sensor is a low cost, portable, reusable, continuous flow polymerase chain reaction (PCR) thermal cycler. Fabrication with low temperature co-fired ceramics (LTCC) can provide a reusable low cost device capable of operating in a wide range of environments The design and manufacture of a prototype continuous flow micro-fluidic PCR device using low temperature co-fired ceramic is presented. Initial modeling of flow characteristics and heat transfer was carried out in SolidWorks™. The prototype device employs resistance heaters below the channels, buried and surface thermocouples for temperature monitoring, and air gaps for thermal isolation.



Materials Science Forum (Volumes 539-543)

Main Theme:

Edited by:

T. Chandra, K. Tsuzaki, M. Militzer , C. Ravindran




K. Moeller et al., "A Prototype Continuous Flow Polymerase Chain Reaction LTCC Device", Materials Science Forum, Vols. 539-543, pp. 523-528, 2007

Online since:

March 2007




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DOI: 10.1109/tcapt.2003.815093

[3] Youngsman, J., Moll, A., Plumlee, D.G. and Schimpf, M. Mini- and MicroChannel Devices in LTCC. Conference: Ceramic Interconnect and Ceramic Microsystems Technology (CICMT), April 10-13, 2005 Baltimore Maryland.

[4] Plumlee, D.G., Fabrication of a Miniature Ion Mobility Spectrometer in low temperature co-fired ceramics. Thesis, MS&E Boise State University (2003).

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