Effect of Calcium Phosphate Glass on Proliferation and Differentiation of MG-63 Cells in HA Scaffolds
This study examined the possibility of synthesis of biphasic calcium phosphate by sintering a mixture of hydroxyapatite and calcium phosphate glass. The effect of the concentration of calcium phosphate glass in a mixture on the proliferation and differentiation of MG-63 preosteoblast-like cells in a hydtoxyapatite scaffold was investigated. The addition of 5 wt% of calcium phosphate glass significantly improved the level of attachment, proliferation and differentiation of MG-63 cells onto the hydroxyapatite scaffolds, particularly when the surface was modified with 2% bovine serum albumin (p<0.05). Under these conditions, type I collagen was expressed and the extracellular matrix was formed by 1 week, and the ALP gene was expressed at 4 weeks.
T. Chandra, K. Tsuzaki, M. Militzer , C. Ravindran
Y. U. Kim et al., "Effect of Calcium Phosphate Glass on Proliferation and Differentiation of MG-63 Cells in HA Scaffolds", Materials Science Forum, Vols. 539-543, pp. 731-736, 2007