Glass-Polydimethysiloxane Hybrid Microthermostat for Restriction Enzyme Digestion

Abstract:

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This paper reports a low-cost microthermostat that is able to maintain a constant temperature necessary for restriction enzyme digestion. Polydimethylsiloxane (PDMS) and Pyrex glass were used to make the microthermostat, because PDMS is a cheap and mass-producible material and both PDMS and glass have very good biocompatibility compared to the more commonly used silicon. A heater made of Au wiring patterned on Pyrex glass was used to control the temperature. A PDMS replica molding technique was used to fabricate a reaction chamber with 3.6 μl capacity. Restriction enzyme digestion was performed by using the fabricated microthermostat and by a conventional method. Then, using gel electrophoresis, we compared results between the microthermostat and conventional methods. It was found that restriction enzyme digestion using the microthermostat required 5 min of heating.

Info:

Periodical:

Materials Science Forum (Volumes 544-545)

Edited by:

Hyungsun Kim, Junichi Hojo and Soo Wohn Lee

Pages:

335-338

DOI:

10.4028/www.scientific.net/MSF.544-545.335

Citation:

Y. J. Ko et al., "Glass-Polydimethysiloxane Hybrid Microthermostat for Restriction Enzyme Digestion ", Materials Science Forum, Vols. 544-545, pp. 335-338, 2007

Online since:

May 2007

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Price:

$35.00

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