Algal Biophysics: Euglena Gracilis Investigated by Atomic Force Microscopy


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Matter produced by organisms is remarkable. Evolutionary optimized properties, e.g. regarding hydrodynamic, aerodynamic, wetting and adhesive behavior, can already be found in the “simplest” forms of organisms. Euglena gracilis, a single-celled algal species, performs tasks as diverse as sensing the environment and reacting to it, converting and storing energy and metabolizing nutrients, living as a plant or an animal, depending on the environmental constraints. We developed a preparation method for atomic force microscopy investigation of dried whole Euglena cells in air and obtained data on whole cells as well as cell parts. Our studies corroborate TEM, SEM and optical microscopy results. Furthermore, we found new features on the pellicle, and set the ground for AFM force spectroscopy and viscoelastic studies on the nanoscale.



Edited by:

Dragan P. Uskoković, Slobodan K. Milonjić and Dejan I. Raković




C. Gruenberger et al., "Algal Biophysics: Euglena Gracilis Investigated by Atomic Force Microscopy", Materials Science Forum, Vol. 555, pp. 411-416, 2007

Online since:

September 2007




[1] C. Sanchez, H. Arribart and M.M. Giraud Guille: Nature Materials Vol. 4 (2005), p.277.

[2] A.E. Clarke and B.A. Stone: Biochim Biophys Acta. Vol. 44 (1960), p.161.

[3] J.R. Sommer: J. Cell Biol. Vol. 24 (1965), p.253.

[4] H.J. Arnott and P.L. Walne: Protoplasma Vol. 64 (1967), p.330.

[5] B.S. Leander and M.A. Farmer: J. Eukaryot. Microbiol. Vol. 47 (2000), p.469.

[6] B.S. Leander and M.A. Farmer: J. Eukaryot. Microbiol. Vol. 48 (2001), p.202.

[7] F. Sbrana, L. Barsanti, V. Passarelli and P. Gualtieri: Atomic force microscopy study on the pellicle of the alga Euglena gracilis, In: From cells to proteins: imaging nature across dimensions, eds: V. Evangelista, L. Barsanti, V. Passarelli and P. Gualtieri, NATO security through science series, Series B: Physics and biophysics, Vol. 3 (2005).


[8] G.F. Leedale: Euglenoid flagellates, Englewood Cliffs, N.J., Prentice-Hall (1967).

[9] E. Meyer, H.J. Hug and R. Bennewitz: Scanning probe microscopy: the lab on a tip (Springer 2003).

[10] W. Kuhlbrandt: Q. Rev. Biophys Vol. 25 (1992), p.1.

[11] P.L. Walne, V. Passarelli, L. Barsanti and P. Gualtieri: Critical Reviews in Plant Sciences Vol. 17 (1998), p.559.


[12] L. Barsanti, V. Passarelli, P.L. Walne and P. Gualtieri: FEBS Letters Vol. 482 (2000), p.247.


[13] S. Hecht, S. Schlaer and M.H. Pirenne: J. Opt. Soc. Am. Vol. 38 (1942), p.196.

[14] D.A. Baylor, T.D. Lamb and K.W. Yau: J. Physiol. Lond. Vol. 288 (1979), p.613.

[15] P. Gualtieri, L. Barsanti andG. Rosati: Arch Microbiol. Vol. 145 (1986), p.303.

[16] E. Marcenko: Cytobiology Vol. 16 (1978), p.485.

[17] D.A. Tirrell (Ed. ): Hierarchical structures in biology as a guide for new materials technology (Nat. Acad. Pr., Washington D.C., USA 1994).

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