The samples of V-N microalloyed mild steel were obtained in duo mill with the normal deformation rate and the normal rolling temperature except final rolling temperature, which is at 780°C, 730°C and 680°C respectively. The tensile test was carried out and the microstructure was observed with optical microscope. It was found that the mechanical properties improved dramatically compared with normal process, the final rolling temperature is more than 900°C. The main reason is the fine grain size and second phase particles. With the final rolling temperature decreasing, the mechanical property further improves until the final rolling temperature of 700°C.