Test Method for Skin Damage of Titania Photocatalyst Nanoparticles In Vitro


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Nanoscale TiO2 is widely used in consumer products like sunscreen and cosmetics. The establishment of damage evaluation test method was attempted to examine the potential neurotoxicity of nanoscale TiO2 to human body skin in vitro model. The emergence amounts of carbon dioxide, which was expected one of the generation products from the skin according to the titania photocatalyst nanoparticles activity under UV / visible light radiation, were identified and measured by the gas analyzer. It was found that it could evaluate the degrees of damage to skin with the photocatalysts activity by using the new evaluation test method considered.



Edited by:

Byungsei Jun, Hyungsun Kim, Chanwon Lee, Soo Wohn Lee




E. Watanabe et al., "Test Method for Skin Damage of Titania Photocatalyst Nanoparticles In Vitro ", Materials Science Forum, Vol. 569, pp. 9-12, 2008

Online since:

January 2008




[1] H. Taoda, E. Watanabe, and K. Kato: Medical Equip. J. Japan, 13, (1995), p.3.

[2] E. Watanabe, H. Taoda, K. Kato, T. Nonami, K. Iseda, and M. Fukaya: Reports of NIRIN, 46(4), (1997), p.227.

[3] M. Fukaya, T. Nonami, E. Watanabe, K. Iseda, M. Maeda, and H. Taoda: Proceedings of the 3 rd International Conference on Ecomaterials, (1997), p.10.

[4] A. Fujishima, K. Hashimoto and T. Watanabe: TiO2 Photocatalysis. Fundamentals and Applications, BKC Inc. Tokyo, (1999).

[5] M. Kato, F. Hamajima, Y. Ito, K. Kubo, K. Hata, M. Nakamura, and S. Shinoda, The 20th Annual Meeting of the Japanese Society for Alternatives to Animal Experiments, (2006).

[6] Japanese Industrial Standard Committee, JIS R 1701-1, Fine ceramics (advanced ceramics, advanced technical ceramics) - Test method for air purification performance of photocatalytic materials - Part 1: Removal of nitric oxides, Japanese Standard Association, (2004).

DOI: https://doi.org/10.3403/30181337

[7] International Organization for Standardization, 22197-1, Test method for air purification performance of semiconducting photocatalytic materials, Part1: Removal of nitric oxide, (2007).

[8] OECD Guidelines for the Testing of Chemicals, (1992), p.404.

[9] L. Thrall, Study links TiO2 nanoparticles with potential for brain-cell damage, Environmental Science & Technology Online News, (2006).

[10] E. Yamamoto, M. Omura, M. Isono, Y. Hirono, S. Mizukami, and K. Takebayasi: J. Ear larynx cervix, 62, (1990), p.337.

[11] E. Watanabe, M. Fukaya, K. Tanaka, and H. Taoda: Proceedings of the 19th international Korea-Japan seminar on Ceramics, (2002), p.509.