Stimuli-Responsive Materials Applied as Carriers of Immobilized Enzymes
Enzymes, immobilized on pH/temperature/ion-responsive carriers, relieve poor contact and could be conveniently recycled as well. Enzymes were immobilized on pH-responsive carriers often by covalent method, which was better than non-covalent method (adsorption). The immobilized enzymes showed slight steric hindrance for enzymatic reaction, but always lose much activity during recycle process. The Km values were often increased significantly via immobilization on temperature/ion responsive carriers, while the immobilized enzymes expressed superior reusability. Both covalent and non-covalent methods were fit for enzymes to be immobilized on temperature-responsive carriers. Non-covalent method (entrapment) was preferential choose for enzymes to be immobilized on ion-responsive carriers. Many drawbacks, such as large activity loss and slow respondence during stimulus occurrence, seriously restrained the effect of the immobilized enzymes. It was supposed to open up special stimuli-responsive materials for enzymes immobilization, and choose proper immobilization methods, correspondingly.
Zhong Wei Gu, Yafang Han, Fu Sheng Pan, Xitao Wang, Duan Weng and Shaoxiong Zhou
Y. Zhang et al., "Stimuli-Responsive Materials Applied as Carriers of Immobilized Enzymes", Materials Science Forum, Vols. 610-613, pp. 1198-1202, 2009