Wood is one kind of renewable natural eco-material. Glued laminated timber (glulam) is an engineered wood product made from sawn lumber lamina glued together in horizontal layers. The application of glulam in the construction structure not only has great environmental value, but also reduces energy consumption and carbon emission of the construction. In this study, we evaluated the effects of different factors on the structural properties of glulam and designed several enhancement modes to reinforce the glulam with FRP (Fiber Reinforced Plastic). Then, we measured the mechanical properties of the glulam specimens in the different enhancement modes and selected the optimal reinforcement mode. The reinforcement method obviously increased the value of MOE and MOR of poplar glulam by 5%-15% and 5%-12% respectively. FRP length of 600mm was the optimal and most economic reinforcement length, and finger joint position of 300mm is the optimal position.