This study aims to study the influence of surface topography on the photocatalytic reaction of TiO2 film surfaces. In this work, a textured TiO2 film surface was fabricated by anodic oxidizing a titanium plate with micro grooves, and its wettabilty was evaluated. Micro grooves were machined on a 3-axis NC control precision machine tool using a single point diamond cutter with a tip radius of several hundred nanometers. Anodic oxidation experiments were conducted by using the self-developed equipment with diluted acetic acid as the electrolyte. It was found that the wettability of the TiO2 film surface textured by microcutting was superior to that textured by polishing under the irradiation environment of ultraviolet rays. The wettability of photocatalytic film surfaces could be improved by increasing the surface area.