A Novel pH-Sensitive Microsphere Composed of CM-Chitosan and Alginate for Sulforaphane Delivery

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Sulforaphane (SF) has been proved to be an effective anticancer agent according to its experiments both in vitro and in vivo. To date, there is few reported method to deliver SF for increasing its bioactivity and stability. In this study, a novel pH-sensitive microsphere composed of water-soluble carboxymethylated chitosan (CMCS) and alginate mixed with sodium sulfate was developed for SF delivery. Swelling studies and release characteristics under different pH values of microspheres were investigated. Then, the release of SF from test microspheres was studied in simulated gastric and segmented intestinal media. It has been found that the SF cumulated release in 5h was increased from 55.89% to 76.73% when the microspheres mixed with sodium sulfate. In addition, the stability of SF embedded in CMCS/alginate microspheres was also significantly improved. Under pH 7.4, free SF had a severe degradation of approximate 100% within 210 min, whereas the change of the SF in microspheres was only a decrease of about 10%. The results suggested that the microspheres of CMCS and alginate could be a suitable pH-sensitive carrier to increase the stability of SF in the segmented intestine.

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Periodical:

Edited by:

Chengming Li, Chengbao Jiang, Zhiyong Zhong and Yichun Zhou

Pages:

539-547

DOI:

10.4028/www.scientific.net/MSF.687.539

Citation:

H. Wang et al., "A Novel pH-Sensitive Microsphere Composed of CM-Chitosan and Alginate for Sulforaphane Delivery", Materials Science Forum, Vol. 687, pp. 539-547, 2011

Online since:

June 2011

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$38.00

[1] J.D. Clarke, R.H. Dashwood and E. Ho: Cancer. Lett Vol. 269 (2008), p.291.

[2] Y. Zhang, P. Talalay, C. Cho and G. Posner: P. Natl. Acad. Sci. USA Vol. 89 (1992), p.2399.

[3] Y. Zhang, T. Kensler, C. Cho, G. Posner and P. Talalay: P. Natl. Acad. Sci. USA Vol. 91 (1994), p.3147.

[4] C. Gerhauser, M. You, J. Liu, R. Moriarty, M. Hawthorne, R. Mehta, R. Moon and J. Pezzuto: Cancer. Res Vol. 57 (1997), p.272.

[5] J.W. Chiao, F. -L. Chung, R. Kancherla, T. Ahmed, A. Mittelman and C.C. Conaway: Sulforaphane and its metabolite mediate growth arrest and apoptosis in human prostate cancer cells (Editorial Academy of the International Journal of Oncology, Athens, GRECE 2002).

DOI: 10.3892/ijo.20.3.631

[6] C. Fimognari, M. Nüsse, R. Cesari, R. Iori, G. Cantelli-Forti and P. Hrelia: Carcinogenesis Vol. 23 (2002), p.581.

[7] L. Gamet-Payrastre, S. Lumeau, N. Gasc, G. Cassar, P. Rollin and J. Tulliez: Anti-Cancer. Drug Vol. 9 (1998), p.141.

DOI: 10.1097/00001813-199802000-00005

[8] D. Gingras, M. Gendron, D. Boivin, A. Moghrabi, Y. Théorêt and R. Béliveau: Cancer. Lett Vol. 203 (2004), p.35.

[9] N. Petri, C. Tannergren, B. Holst, F. Mellon, Y. Bao, G. Plumb, J. Bacon, K. O'Leary, P. Kroon and L. Knutson: Drug. Metab. Dispos Vol. 31 (2003), p.805.

[10] D. Van Eylen, I. Oey, M. Hendrickx and A. Van Loey: J. Agr. Food. Chem Vol. 55 (2007), p.2163.

[11] D. Do, S. Pai, S. Rizvi and M. D'Souza: Int. J. Pharm Vol. 386 (2010), p.114.

[12] L. Zhang, J. Guo, X. Peng and Y. Jin: J. Appl. Polym. Sci Vol. 92 (2004), p.878.

[13] Y. Shao, B. Zhu, J. Li, X. Liu, X. Tan and X. Yang: Mat. Sci. Eng. A-struct: C Vol. 29 (2009), p.936.

[14] F. -L. Mi, Y. -C. Tan, H. -F. Liang and H. -W. Sung: Biomaterials Vol. 23 (2002), p.181.

[15] T. Chandy and C. Sharma: Artif. Cell. Blood. Sub Vol. 18 (1990), p.1.

[16] Y. Murata, S. Toniwa, E. Miyamoto and S. Kawashima: Int. J. Pharm Vol. 176 (1999), p.265.

[17] Van der Lubben, I. M., J.C. Verhoef, G. Borchard and H.E. Junginger: Eur. J. Pharm. Sci Vol. 14 (2001), p.201.

[18] R.A.A. Muzzarelli: Carbohyd. Polym Vol. 8 (1988), p.1.

[19] H. Liang, C. Li, Q. Yuan and F. Vriesekoop: J. Agr. Food. Chem Vol. 55 (2007), p.8047.

[20] Y. -H. Lin, H. -F. Liang, C. -K. Chung, M. -C. Chen and H. -W. Sung: Biomaterials Vol. 26 (2005), p.2105.

[21] X. Liu, W. Xue, Q. Liu, W. Yu, Y. Fu, X. Xiong, X. Ma and Q. Yuan: Carbohyd. Polym Vol. 56 (2004), p.459.

[22] X. Li and B.R. Jasti: Design of controlled release drug delivery systems (McGraw-Hill, New York 2006).

[23] G.S. Macleod, J.H. Collett and J.T. Fell: J. Control. Release Vol. 58 (1999), p.303.

[24] G.P. Basten, Y. Bao and G. Williamson. J. Life Sci Vol. 23 pp.1399-1404.

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