Reconstituted Extracellular Matrix Improve Osteoblastic Differentiation onto Titanium Surfaces


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Electrospinning technique is an efficient processing method to manufacture micro-and nanosized fibrous structures by electrostatic force for different applications. In biomaterial field, electrospinning technique has been successfully utilized to prepare new drug delivery materials and tissue engineering scaffolds. Fiber mats of biodegradable polymers having a diameter in the nanoto submicro-scale can be considered to mimic the nanofibrous structure of native extracellular matrix (ECM). Native extracellular matrix, constituted of proteins and polysaccharides improving cells growth in its nanofibrous porous structure, controls not only the cell phenotype, but the whole structure of the biological tissues. In the present study we investigated the effect of electrospun reconstituted collagen fibers onto metals for oral implants devices manufacturing as far as the osteoblastic differentiation potential of stem cells and cytofunctionality of osteoblasts in-vitro. The cells cultured onto titanium samples coated with ECM constituents showed faster osteoblastic differentiation and more efficient deposition of mineralized matrix in comparison with those onto uncoated substrates.



Materials Science Forum (Volumes 706-709)

Main Theme:

Edited by:

T. Chandra, M. Ionescu and D. Mantovani




L. Rimondini et al., "Reconstituted Extracellular Matrix Improve Osteoblastic Differentiation onto Titanium Surfaces", Materials Science Forum, Vols. 706-709, pp. 584-588, 2012

Online since:

January 2012




[1] Hemmat S, Lieberman DM, Most SP. An introduction to stem cell biology. Facial Plast Surg. 2010 Oct; 26(5): 343-9.


[2] Mizuno H: Adipose-derived stem cells for tissue repair and regeneration: ten years of research and a literature review. J Nippon Med Sch ; 2009 Apr; 76(2): 56-66.


[3] Brown SA, Levi B, Lequex C, Wong VW, Mojallal A, Longaker MT. Basic science review on adipose tissue for clinicians. Plast Reconstr Surg. 2010 Dec; 126(6): 1936-46.


[4] Rimondini L, Mele S. Stem cell technologies for tissue regeneration in dentistry. Minerva Stomatol. 2009 Oct; 58(10): 483-500.

[5] Tuzlakoglu K, Reis RL. Biodegradable polymeric fiber structures in tissue engineering. Tissue Eng Part B Rev. 2009 Mar; 15(1): 17-27.


[6] Parma B. Patent 2002 n8 WO 02097990.

[7] Foltran I. Foresti E. Parma B. Sabatino P. Roveri N. Novel Biologically Inspired Collagen Nanofibers Reconstituted By Electrospinning Method. Macromolecular Symposia; 2008, 269, 111-118.


[8] Lee, KY et al. Electrospinning of polysaccharides for regenerative medicine Advanced Drug Delivery Reviews 2009; 61: 1020–1032.


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