Authors: Li Gen Wu, An Na Wang, Lin Lu Jia, Xiu Ling Li, Yu Dan Sun, Xiang Ying Xu
Abstract: Two fractions, such as Fr1-3 and Fr2-2, were obtain after separating and purifing crude kiwifruit glycoprotein by using Sepharose Cl-6B gel column and DEAE Sepharose Cl-6B ion exchange column. In the UV and Infrared Spectroscopy of Fr1-3 and Fr2-2, the characteristic absorption peaks of sugar and protein were both found.The molecular weight of Fr1-3 is 30497,the molecular weight of Fr2-2 is 28567. Both of Fr1-3 and Fr2-2 have 17 amino acids, However, the amino acid content of them is different.
1730
Authors: Bin Wang, Jia Hui Ma, You Le Qu, Xian Wang, Li Li
Abstract: In order to evaluate the high-value application of squid by-products yielded hydrolysate, the process of preparation and purification technology, chemical composition and in vitro antioxidant activity of the hydrolysate were investigated. The optimal conditions of papain hydrolysis were obtained by single-factor experiments and orthogonal test with the DPPH• scavenging ratio as index, amino acid composition was analysed by automatic amino acid analyzer, the hydrolysate was isolated with a Sephadex G-25 column. Based on single-factor experiments, the hydrolysate with the DPPH• scavenging ratio being 53.96 % was gained under the optimal condition of enzymolysis temperature of 45 °C, enzymolysis time of 3 h, total enzyme dose of 1.2 %, and the pH value of 7. The protein content of the hydrolysate reached up to 17.53 %, and the essential amino acids were accounted for 51.06 % of total amino acids. The largest content amino acid was glutamic acid, which accounted for 10.74 % of total amino acids. Compared with the amino acid profiles recommended by FAO/WHO, the quality of the protein hydrolysate was high, as it was rich in essential amino acids, including isoleucine, leucine, lysine, methionine and cystine, threonine, tryptophan, and valine, which covered 88 %-100 % of the FAO/WHO recommended. The hydrolysate was divided into three fractions (F1-F3) using a Sephadex G-25 column, the F1 possessed the highest antioxidation activity with the reducing power, •OH and DPPH• scavenging ratio being 0.236, 18.13 % and 63.85 % at the concentration of 5 mg/mL. Compared with the retention time of the reduced glutathione chromatomap, the relative molecular mass of F1 was higher than 307, F2 and F3 was lower than 307. The result revealed that the protein hydrolysate from squid by-products by papain had strongly antioxidant capacity in vitro and high nutrition, and this finding provided a new way of advanced exploitation of squid scrap resources.
198
Authors: Xin Zhao, Wen Ying Zhu
Abstract: Abstract. Amino-silicone Softener Was Synthesized by the Hydrosilylation Reaction, with Dimethylaminoethyl Acrylate, Polymethylhydrosiloxane as the Raw Materials, and Chlorioplatinic Acid as the Catalyst. the Copolymer Was Analyzed Using Ir Spectroscopy, then Emulsified it and Used in the Linen Fabric. the Results Showed that the Amino Group Can Be Introducted to Silicone Side Chains through Hydrosilylation that Dimethylaminoethyl Acrylate Grafted with Polymethylhydrogensiloxane, Obtained with the Softness of the Amino-modified Silicone Oil.
390
Authors: Tao Zhang, Yan E Luo, Dai Di Fan, Lei Guo, Ting Zhen Mu
Abstract: Amino acids, the basic unit of protein molecules, are closely related to some important biological activities and they affect cell growth and metabolism directly or indirectly. Previous studies showed that adding amino acids can improve the productivity of human-like collagen (HLC). Thus, we analyzed the amino acids metabolism during the fermentation process. The results of metabolic flux analysis of amino acids implied that the cell growth and production of HLC were active as long as the free amino acids in the medium were adequate. This suggested control the concentration of amino acids could improve cell growth and human-like collagen production.
1055
Authors: Jun Xue, Ya Jun Wang, Ming Yi Rao, Guo Tian Luo
Abstract: A new L-amino acid tailed porphyrins and its Zinc(Ⅱ) complex were synthesized by L-amino acid and 5-(2-ethoylphenyl)-10, 15,20-triphenylporphyrin.FAB-MS, UV-vis, IR element analysis and chemical analysis were used to determine the structures of the porphyrin and zinc(Ⅱ) complex.
2398