Effects of Silver Nanoparticles on the In Vitro Culture and Differentiation of Human Bone Marrow-Derived Mesenchymal Cells

Abstract:

Article Preview

The antibacterial properties of silver nanoparticles may be able to prevent inflammation around an implant when placed on the surface of the dental neck. The purpose of this study was to culture silver nanoparticles on human bone marrow stem cells in vitro to determine their effects on cell differentiation and to assess their biocompatibility. Silver nanoparticles were deposited on titanium foil implant surfaces using ion sputtering, and adult bone marrow mesenchymal stem cells were cultured on those surfaces to determine the effect of the silver nanoparticles on cell viability, suitability for subculture, proliferation and differentiation. The results indicated that the silver nanoparticles were biocompatible, allowing cell proliferation and differentiation of osteoblasts, but interfered with differentiation into chondrocytes and adipocytes.

Info:

Periodical:

Edited by:

Yu Xun Wang, Gui Chun Huang and Linqing Luo

Pages:

1307-1312

Citation:

X. Q. Yu and L. X. Xu, "Effects of Silver Nanoparticles on the In Vitro Culture and Differentiation of Human Bone Marrow-Derived Mesenchymal Cells", Materials Science Forum, Vol. 852, pp. 1307-1312, 2016

Online since:

April 2016

Export:

Price:

$38.00

[1] Afya Sahib Diab Al-Radha, Abhi Pal, Andre Philip Pettemerides, Howard F: journal of dentistry, 2012: 40, 989.

DOI: https://doi.org/10.1016/j.jdent.2012.08.006

[2] Heydenrijk K, Meijer HJ, Van Der Reijden WA, Raghoebar GM, Vissink A, Stegenga B.: The International Journal of Oral & Maxillofacial Implants 2002; 17: 829.

[3] Claffey N, Clarke E, Polyzois I, Renvert S: Journal of Clinical Periodontology 2008; 35: 316.

[4] Klinge B, Hultin M, Berglundh T. Peri-implantitis: Dental Clinics of North America 2005; 49: 661.

DOI: https://doi.org/10.1016/j.cden.2005.03.007

[5] Lang NP, Berglundh T. Periimplant: Journal of Clinical Periodontology 2011; 38(Suppl. 11): 178.

[6] Crewlich C, Kittler S, Epple M, Muhr G, Koller M.: Langenbecks Arch Surg. 2009, 394(3): 495.

[7] Hussain SM, Hess KL, Gearhart JM, Geiss KT, Schlager JJ: Toxicol In Vitro, 2005, 19: 975.

[8] HsinYH, Chen CF, Huang S, Shih TS, Lai PS, Chueh PJ: Toxicol Lett., 2008, 179 (3): 130-139.

[9] Kawata K, Osawa M, and Okabe S.: Environ. Sci. Technol. 2009, 43: 6046-51.

[10] AshaRani PV, Prakash Hande M, and Valiyaveettil S: BMC Cell Biology, 2009, 10: 65-79.

DOI: https://doi.org/10.1186/1471-2121-10-65

[11] AshaRani PV, Low Kah MG, Hande MP: ACS nano. 2009, 3: 279-290.

[12] Kumari M, Mukherjee A, Chandrasekaran N: Sci Total Environ, 2009, 407(19): 5243~5246.

[13] AshaRani PV, Mun GLK, Hande MP, Valiyaveettil S: ACS NANO, 2010, 3(2): 279~290.