Building Blocks for Artificial Extracellular Matrices Based on Cross-Linkable Polysaccharide and Glycosaminglycan Sulfates


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Natural sulfated glycosaminoglycans (GAGs) play a crucial role as components of the extracellular matrix (ECM). They participate in the regulation of important cellular functions including cell growth, differentiation and signalling. The generation of artificial ECM mimicking selected functions of the native ECM is a promising approach to improve the biological acceptance of materials which are in direct contact to living tissue. In this context we developed synthesis routes for polysaccharide and GAG derivatives bearing both bioactive sulfate and reactive (meth) acrylate functions of different degrees of substitution within the sugar repeating unit. In addition, we studied the photochemically initiated cross-linking of these biopolymer derivatives to form biodegradable hydrogels usable as coatings for biomaterials or scaffolds in tissue engineering.



Main Theme:

Edited by:

C. Sommitsch, M. Ionescu, B. Mishra, E. Kozeschnik and T. Chandra




J. Becher et al., "Building Blocks for Artificial Extracellular Matrices Based on Cross-Linkable Polysaccharide and Glycosaminglycan Sulfates", Materials Science Forum, Vol. 879, pp. 1270-1275, 2017

Online since:

November 2016




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[1] M. Schnabelrauch, D. Scharnweber, J. Schiller, Sulfated glycosaminoglycans as promising artificial extracellular matrix components to improve the regeneration of tissues, Curr. Med. Chem. 20 (2013) 2501-2523.


[2] E. Ruoslahti, E. Engvall, Complexing of fibronectin glycosaminoglycans and collagen. Biochim. Biophys. Acta 631(1980) 350–358.


[3] B.P. Purcell, I.L. Kim, V. Chuo, T. Guenin, S.M. Dorsey, J.A. Burdick, Incorporation of sulfated hyaluronic acid macromers into degradable hydrogel scaffolds for sustained molecule delivery, Biomater. Sci., 2 (2014) 693-702.


[4] V. Hintze, S. Moeller, M. Schnabelrauch, S. Bierbaum, M. Viola, H. Worch, D. Scharnweber, Modifications of Hyaluronan Influence the Interaction with Human Bone Morphogenetic Protein-4 (hBMP-4), Biomacromolecules 10 (2009) 3290-3297.


[5] A. van der Smissen, V. Hintze, D. Scharnweber, S. Moeller, M. Schnabelrauch, A. Majok, J. C. Simon, U. Anderegg, Growth promoting substrates for human dermal fibroblasts provided by artificial extracellular matrices composed of collagen I and sulfated glycosaminoglycans, Biomaterials 32 (2011).


[6] R. Mhanna, A. Kashyap, G. Palazzolo, Q. Vallmajo-Martin, J. Becher, S. Möller, M. Schnabelrauch, M. Zenobi-Wong, Chondrocyte culture in three dimensional alginate sulfate hydrogels promotes proliferation while maintaining expression of chondrogenic markers, Tiss. Engn. 20 (2014).


[7] S. Reichelt, J. Becher, J. Weisser, A. Prager, U. Decker, S. Möller, A. Berg, M. Schnabelrauch, Biocompatible polysaccharide-based cryogels, Mater. Sci. Engn. C35 (2014) 164-170.


[8] J. Becher, S. Möller, M. Schnabelrauch, Phase-transfer-catalyzed synthesis of highly acrylated hyaluronan, Carbohydr. Polym. 93 (2013) 438-441.