Photolithography of 3D Scaffolds for Artificial Tissue
The present study is focused on the design and fabrication of novel functional 3D-hydrogel scaffolds for regenerative medicine. In order to critically analyzing the effect of the microarchitecture of 3D scaffolds for driving the cellular fate and diffusion of progenitor stem cells we have fabricate a number of scaffolds with different geometry, stiffness and composition. The physical characteristics of the scaffold determine indeed, as well the biochemical factors, the fate of the cells. We use an innovative composite material consisting of hydrogel with different molecular weight and with suitable accordion-like and woodpile structures in order to tailor stiffness and elasticity conferred to the final structure.These novel 3D bioinspired scaffolds were obtained by both single- (1PP) and two-photon polymerization (2PP) processing. In particular, 2PP scaffolds represent a great advantage with respect to previous achievements based on traditional methods. 3D-structures were fabricated with lateral resolution of some microns, allowing an advanced control of pore microarchitecture of defined tensile strength, and the inclusion of albumin microspheres with various functionalities. The morphological, biochemical and functional characteristics are discussed. Moreover, the effects of the structured hydrogel scaffolds on the proliferation and differentiation of adult stem cells is analyzed in view of the fabrication of portion of contractile cardiac muscle to be obtained In Vitro.
C. Sommitsch, M. Ionescu, B. Mishra, E. Kozeschnik and T. Chandra
P. Prosposito et al., "Photolithography of 3D Scaffolds for Artificial Tissue", Materials Science Forum, Vol. 879, pp. 1519-1523, 2017