Gold Nanoflowers and Gold Nanospheres as Labels in Lateral Flow Immunoassay of Procalcitonin

Abstract:

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In this study, new types of nanogold labels for lateral flow immunoassay of model antigen procalcitonin based on photometric and surface-enhanced Raman scattering methods of detection were obtained. The linear range of procalcitonin determination applying gold nanoflowers as a label was between 0.5 and 10 ng/mL. The limit of photometric detection was achieved at 0.1 ng/mL that was five times lower than the sensitivity of traditional lateral flow immunoassay with spherical gold nanoparticles as a label. In addition, the conjugate of polyclonal antibodies against procalcitonin with spherical gold nanoparticles labeled with 4-mercaptobenzoic acid as Raman reporter molecule was prepared and used as an immunoprobe in lateral flow immunoassay based on surface-enhanced Raman spectroscopy to improve assay sensitivity.

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Periodical:

Edited by:

Alexander Gusev, Tatiana Dyatcheck and Anna Godymchuk

Pages:

47-53

DOI:

10.4028/www.scientific.net/NHC.13.47

Citation:

K. V. Serebrennikova et al., "Gold Nanoflowers and Gold Nanospheres as Labels in Lateral Flow Immunoassay of Procalcitonin", Nano Hybrids and Composites, Vol. 13, pp. 47-53, 2017

Online since:

January 2017

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$35.00

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