Specific Immobilization of Streptavidin on Mixed Self-Assembled Monolayers as Mixing Ratio
We report on the distribution of mixed self-assembled monolayers (SAMs) composed of biotinylated and diluent alkylthiolates for streptavidin immobilization. Two thiol derivatives, 11-mercapto-1-undecanol (MUOH) and 11-mercaptoundecanoic-(8-biotinylamido-3,6-dioxaoctyl) amide (MBDA), were employed for mixed SAM. These thiols formed self-assembled monolayer without local domain, and streptavidins were immobilized onto biotinylated gold surface without nonspecific binding. In order to find the optimized condition of immobilization of streptavidin, we controlled the mixing ratio of two kind thiols by colorimetric detection assay, and the immobilization was characterized by atomic force microscopy (AFM), scanning tunneling microscopy (STM), and ellipsometer.
Chunli BAI, Sishen XIE, Xing ZHU
J. H. Park et al., "Specific Immobilization of Streptavidin on Mixed Self-Assembled Monolayers as Mixing Ratio", Solid State Phenomena, Vols. 121-123, pp. 495-498, 2007