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Selection of a mutant strain Streptococcus equi SH-109 and optimization of culture conditions for the production of high molecular weight hyaluronic acid Jiancheng Feng1 , Ronghu ZHANG 1,2﹡ 1 Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, Haikou Hainan,570228, China 2Farm Produce Processing and Design Research Institute, Hainan Academy of Agricultural Sciences , Haikou, Hainan, China 570110 ﹡corresponding author fjc197228@126.com Keywords: Streptococcus equi; hyaluronic acid; high molecular weight; culture conditions Abstract.
Materials and methods Bacterial Strain Streptococcus equi SH-0 was preserved by Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, as a freeze-dried culture in ampoules and maintained at 4°C.
Pulicherla:International Journal of Advanced Science and Technology,Vol.60, (2013), p.83-96
[8] HJ Gao, GC Du, JChen: World Journal of Microbiology & Biotechnology, Vol.10(2006),P.399-408

Equivalent Load Identification Algorithm Based on Least-squares in Frequency Domain Cheng Wanga and Jin Goub College of Computer Science and Technology, HuaQiao University, Xiamen 361021, China awangcheng@hqu.edu.cn, bgoujin@gmail.com Keywords: Equivalent Load; Least-Squares; Transfer Function; Frequency Load Identification.
We adopted Hypermesh to model, meshed, set materials, and imported them into VAone.
Acknowledgements This work was financially supported by the National Natural Science Foundation of China (Grant No. 61103170).
International Journal of Applied Electromagnetics and Mechanics, 2010, p.1001-1008
Journal of Sound and Vibration,2009,319, p. 869-884.

Materials and Methods Wood density Samples.
Forest Products Journal, 44(5): 43–48
Journal of Forestry Research, 15(4): 261-267
Forest Science, 51(4):357–371
Wood and Fiber Science, 39(2): 352–363

In order to use yeast , several strains of high yield of ethanol Saccharomyces cerevisiae are separated through experimental, choosing one strain which has unique taste in fermentation, another strain has strong ability of alcohol tolerance, the two strain are used to make protoplasts, which lay the foundation for use the two yeasts in the further[2]. 1.material and method 1.1 material Strain: the yeast from isolation ,the NO. is T67, T13 .
Acknowledgements This research is supported by Grant BS2012SW001 from National Natural Science Foundation of Shan Dong Province.
References: [1] Zhao chun hai kan zhen rong Study on Polysaccharide from Edible and Medical Fungus FaJiao KejiTongXun 2008, 37（2）: 14-16 [2] WANG Yan; ZHANG Wenxue; GUO Hongcheng Protoplast fusion of alcoholic fermentation yeast and pigmented yeast China Brewing, 2010(1): 68-70 [3] Zhou hai xia,Kan zhen rong, Zhang shuang feng, Protoplast Fusion of Bacillus subtilis Produced α-ALDC journal of Hebei University(Natural Science Edition) 2004, 24(3): 288-292 [4] T.
Harrison Factors affecting mango (Mangifera indica L.) protoplast isolation and culture Scientia Horticulturae, 2011, 130(1): 214-221 [7]Anika Nadja Sabine Prange, Melanie Bartsch, etal.Regeneration of different Cyclamen species via somatic embryogenesis from callus, suspension cultures and protoplasts Scientia Horticulturae, 2010,125(3) : 442-450 [8]Sushruti Sharma, Debabrata Sarkar, Suman Kumar Pandey, etal.Stoloniferous shoot protoplast, an efficient cell system in potato for somatic cell genetic manipulations Scientia Horticulturae, 2011, 128(2) :84-91 [9]Hideki Aoyagi Application of plant protoplasts for the production of useful metabolites Biochemical Engineering Journal, 2011, 56（1）: 1-8 [10]Olav Keerberg, Hiie Ivanova, Hille Keerberg, eral.

Journal of Hazardous Materials B, 2006 137 152-156
Progress in Energy and Combustion Science，2003 29(5) 381-405
Journal of Combustion Science and Technology, 2000 6(1) 47-50 (in Chinese).

Besides, there is another small part of basalt provides metallogenic material to bauxite.
Journal of Geochemical Exploration, Vol. 133(2013), P.108
Journal of palaeogeography,Vol.16(1),P.9(in Chinese)
Geological science and technology information, Vol.32(1),P.4(in Chinese)
Geological science and technology information, Vol.32(1),P.47(in Chinese).

Representation of Developable Surfaces and Achievement of the Algorithm Min Zhou1,a, Zhenglin Ye1,b, Guohua Peng1,c, Junqing Yang2 and Hongchan Zheng1 1College of Science, Northwestern Polytechnical University, Xi'an 710072, China 2Department of Computer Engineering, Xi'an Aerotechnical College, Xi'an 710077, China ayjqfive@163.com, byezhenglin@sina.com, cpenggh@nwpu.edu.com Keywords: Developable surface, Duality, Control plane, Control parameter, Subdivision rule, Triangle-B spline, Algorithm Abstract.
Therefore, many products that use sheet metal, leather, paper and other similar flexible materials are formed using developable surfaces.
Acknowledgement This work is Supported by Graduate Starting Seed Fund of Northwestern Polytechnical University (z200571); Shanxi Province Natural Science Foundation (2004A03).
Aumann: Journal of Theoretical Graphics and Computing, (1994) No.7, pp.12-26
Maekawa: Journal of Ship Research, Vol.42 (1998) No.3, pp.207-215

Yang, International Journal of Heat and Mass Transfer, 92 (2016) 158–172
Owen, International Journal of Thermal Sciences,47 (2008) 1276-128
Houat, IOP Conference Series: Materials Science and Engineering, 186 (2017) 012036.

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Acknowledgements Funded by the National Natural Science Foundation (Grant No. 50365001),Guangxi Scientist &technology department project (Grant No. 0235008-5),Guangxi Manufacture system &advancing Manufacture technology key laboratory project (Grant No. 07109008_021_K)Guangxi science & technology development plan(Grant NO.0992002-18) and Guangxi graduate students innovation plan (No.105930901023） References [1] R.R.
Li: Experimental Research on Influence Factor of Cutting-force of Sugarcane Harvest Machine．Journal of Agricultural Mechanization Research, 2006.7 [2] L.
Zhang Mechanics of Materials (West North Industrial University Publications, Xian 2005.2)
Li: Virtual prototype technology and its application in agriculture mechanical design ”, Journal of Agricultural Mechanization Research, Apr.2004 [6] MSC.software,corporation:Using ADAMS/Solver, Version2005,(Tsinghua University Publications, Beijing 2004)