Papers by Author: Bing Li

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Authors: Quan Yi Fu, Xu Cong Yu, Lin Li, Guo Qin Liu, Bing Li
Abstract: Brewers’ spent grain (BSG) is the most common by-product of the brewing industry, contains about 16.8% cellulose, 28.4% non-cellulosic polysaccharides and 27.8% lignin, which is a good raw material for industrial production of soluble dietary fiber (SDF).The antioxidant activity of crude dietary fiber (CDF) and grade dietary fiber (GDF) isolated from BSG was investigated in vitro. The scavenging hydroxyl radical capability of CDF and GDF was respectively reached to15.4% and 69.2% at the concentration of 0.1mg /mL. DF concentration from 0.02mg /mL to 0.1m g /mL was used. The scavenging capability of CDF and GDF to superoxide anion radical was increased from 10.68% and 12.33% to 35.78% and 78.39%. Both of the two DF showed some effect in scavenging superoxide radical and 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) radical with the concentration increase. The result showed that BSG DF can be used as a good additive with some functionality.
Authors: Ling Chen, Xian Liang Song, Jian Dong Ye, Bing Li
Abstract: The forming of Al2O3 ceramics was carried out by the colloidal in-situ consolidation using modified starch in this work. The effects of the modified starch content in the alumina suspensions on their rheological behavior, consolidation, microstructure and properties of the green bodies were investigated. The different shape and high relative density of ceramic green bodies were achieved by this method. The results indicated that the alumina suspensions with modified starch were easier to cast due to their low viscosity and high fluidity. After drying, the linear shrinkage of the green bodies was low, ranging between about 1.5% and 2.2% and their bulk density was higher than 57% of the theoretical density. The maximum bending strength of the dried green bodies reached to 8.2 MPa and the microstructure of the green bodies was uniform with a narrow unimodal distribution of pore size. It is concluded that the colloidal in-situ consolidation casting using modified starch can perform near-net shaping of ceramic components with high-performance and it is a potential forming technique for ceramics.
Authors: Yang Deng, Yan Rui Liang, Lin Li, Bing Li, Jian Yu Su
Abstract: A loop-mediated isothermal amplification (LAMP) method for rapid detection of various staphylococci strains and associated antibiotic resistance determinant had been developed and evaluated in this study. Six primers, including outer primers, inner primers and loop primers, were specially designed for recognizing eight distinct sequences on four targets: 16Sr RNA, femA, mecA, and orfX. Twenty-seven reference strains, including various species of gram-negative and-positive isolates, were included in this study to evaluate and optimize LAMP assays. The optimal reaction condition was found to be 65°C for 45 min, with detection limits at 100 fg DNA/tube and 10 CFU/reaction for 16S rRNA, 100 fg DNA/tube and 10 CFU/reaction for femA, 1 pg DNA/tube and 100 CFU/reaction for mecA, 10 DNA/tube and 10 CFU/reaction for orfX, respectively. Application of LAMP assays were performed on 166 various types of staphylococci isolates, the detection rate of LAMP assays for the 16Sr RNA, femA, mecA, and orfX was 100% (166/166), 98.5% (64/65), 94.3% (66/70), and 98.6% (69/70) and the negative predictive value (NPV) was 100%, 98.1%, 92.3%, and 92.7% respectively; with a 100% positive predictive value (PPV) for all three targets. In conclusion, LAMP assays were demonstrated to be useful and powerful tools for rapid detection of various staphylococci strains.
Authors: Xu Cong Yu, Lin Li, Quan Yi Fu, Bing Li
Abstract: In this article, investigation on the effect of microwave treatment on the catalysis of papain had been carried out. According to the results, the thermal effect was found to be the main factor to cause inactivation of enzyme. The kinetic parameters and the fluorescence spectroscopy implied that the microwave processing had changed the conformation of the enzyme molecules and thus affected its activity. The results also showed that the microwave treatment had not changed the optimum pH value of the enzyme catalysis, and the inactivation rate of the enzyme was inversely proportional to the concentration of the enzyme. In addition, a certain concentration of the disodium EDTA media had been found to effectively protect the enzyme activity in microwave processing.
Authors: Ling Chen, Xiao Xi Li, Lin Li, Bing Li
Abstract: Colon-targeting drug delivery systems (CDDSs) are employed to improve the bioavailability of protein and peptide drugs through the oral route. So it is important to prepare the drug carriers for oral CDDS. In this study, the Enzyme-Resistant starch (RS) was studied for use as a vehicle in oral colon-targeting drug delivery. The characteristics of RS powders were investigated by X-ray diffraction, polarizing microscopy, DSC and SEM, and their film were examined by enzymatic digestion test. The results showed that RS could be a promising film-former for pharmaceutical coatings, having good stability to enzymatic digestion. Furthermore, a novel peroral formulation using RS coating and bovine serum albumin as a model drug was studied for colon-specific drug delivery in vitro. Drug release studies have shown that RS coating could delivery the drug to the colon and the release rate in simulated colonic fluids was dependent on the biodegradation of RS and its coatings. It is indicated that the RS coated tablet is a potential system for oral CDDS.
Authors: Lei Zhao, Guo Qin Liu, Bing Li, Lin Li
Abstract: Ultrasonic was employed to improve the functional properties of wheat gluten. The results showed their solubility, emulsifying capacity and emulsion stability index of ultrasonically treated wheat gluten gradually increased as the ultrasonic power and treatment time increase, when ultrasonic treated for 20min at 240W, the solubility, emulsifying capacity and emulsion stability reached the maximum, then the solubility slightly decreased when the ultrasonic power exceeded 240W. However, the forming capacity and foam stability of ultrasonically treated gluten samples gradually increased with ultrasonic power and treatment time increasing. Gel Permeation Chromatography (GPC) observations revealed that the molecular weight of gluten decreased as the ultrasonic power increased, implying that the molecular of wheat gluten was broken by ultrasonic. Lower molecular weight of wheat gluten showed better functional properties.
Authors: Bing Li, Shou Li Dong, Xiao Ling Xie, Zhen Bo Xu, Lin Li
Abstract: A new carrier-free cross-linked aggregates of cellulase (CLEAs-C) via precipitation with ammonia sulfate and cross-linking with glutaraldehyde was prepared. Efficient enzyme activity was obtained when cellulase and glutaraldehyde concentration was 50mg/mL and 3% (v/v) respectively. The cross-linking time and temperature were also important parameters for immobilization. Optimal temperature and pH of the CLEA–C were evaluated. The CLEAs-C displayed good stabilities, after stored at 4○C for 28 days storage, the CLEAs retained more than 80% initial activities.
Authors: Xiao Ling Xie, Bing Li, Zhi Qing Wu, Shou Li Dong, Lin Li
Abstract: The cellulase was immobilized onto magnetic chitosan microspheres carrier as cross-linked enzymes aggregates (CLEAs). It was precipitated with 95% saturation ammonia sulfate and cross-linked with 3% (v/v) glutaraldehyde. Efficient enzyme activity about 50.6% was obtained when cellulase concentration was 1.0mg/mL after cross-linking for 7 h at 30C. The CLEAs was advantageous on stabilities and magnetic responsiveness for separation.
Authors: Xiao Xi Li, Ling Chen, Bing Li, Lin Li
Abstract: Zirconia nanopowders were prepared in the ultrasonic field by the sol-gel method and the sonochemical effect on the structure of zirconium hydroxide and the zirconia nanopowder properties were systematically investigated in this work. Ultrasound was introduced into the different stages of the synthesis of zirconia nanopowders in sol-gel reaction system, and zirconium hydroxides and the zirconia nanopowders with different properties were obtained. The results indicated that ultrasonic cavitation could not only disaggregate the agglomerates of zirconia colloidal particles but also reduce the amount of coordinated H2O, free H2O and free hydroxyl groups of the zirconium hydroxide colloidal particles, thus effectively preventing the formation of hard agglomerates in zirconia powders. Moreover, the effects of different ultrasonic output powers and treatment cycles on the structure and properties of ZrO2 nanopowders were studied by TEM, XRD and SAXS. Zirconia nanopowders with an extremely small crystallite size (10.3 nm) and a narrow size distribution were yielded with 520 W ultrasound for 6 treatment cycles on the formation period and 600 W ultrasound for 2 treatment cycles on the washing period. It is concluded that the ultrasonic field is a potential method for nanopowder preparation.
Authors: Hong Jia Lin, Lin Li, Ying Juan Tian, Xia Zhang, Bing Li
Abstract: Brewer’s spent grain (BSG) rich in proteins is the main by-products in brewery. BSC proteins were extracted and hydrolyzed by Alcalase. The effects of hydrolysis time, temperature, pH and amount of enzyme added on the peptide yield were investigated. Inhibition of BSG protein hydrolysate against α-glucosidase was evaluated in vitro. BSG protein hydrolysate at 4.0 mg/mL showed the inhibition of 21.42% against α-glucosidase. The BSG protein hydrolysate after ultrafiltration purification exhibited much higher inhibition than that without ultrafiltration purification. The inhibition of BSC protein hydrolysate fraction with molecular weight fraction less than 5 kDa was 56.41%.
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