Papers by Author: G. Mayer

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Authors: H.F. Hildebrand, N. Blanchemain, G. Mayer, Y.M. Zhang, O. Melnyk, M. Morcellet, B. Martel
Abstract: In order to improve the tissue integration and subsequently the long-term maintenance, the implant surface can be modified by mechanical, physical, chemical or biological functionalization. In this way, the surface becomes biologically active by further grafting of biomolecules. Two principal concepts are considered for materials functionalization. (i) The Drug Delivery Systems (DDS) where the bioactive molecules goes to the target. (ii) The grafting of the bioactive compounds on small strongly bound spacer molecules. In this system, the target goes to the bioactive molecules. These techniques provide promising outlooks for any polymeric or ceramic scaffold used in tissue engineering for the construction of whole artificial and functional organs.
Authors: N. Blanchemain, G. Mayer, M. Traisnel, Y. Setti, H.F. Hildebrand
Abstract: PE is manufactured by Transysteme with the method of thermocompression of PE powder. This company irradiates PE by YAG LASER. We compared the physical behavior of both samples. The DSC reveals a high and similar crystallinity of PE, before and after YAG LASER treatment. Weattability between PE and Li-PE are similar at about 37 mJ/cm². The difference appears with respect to the surface profile and composition: the roughness is 0.20 µm for PE and 0.29µm for Li- PE. XPS reveals many traces of stripping agents on PE. YAG Laser produces a surface cleaning effect. Biological tests reveal a non-toxicity of the polyethylene powder, an improved proliferation and vitality of L132 cells on Li-PE with respect to untreated PE. We have a 4-fold improvement of proliferation and a 2-fold improvement of vitality on Li-PE with respect to PE. As to morphology and cell adhesion behavior, we have no differences between both samples. Thus the irradiation process of PE by YAG LASER improved the biological behaviors of this polymer due to changes in roughness and to surface cleaning.
Authors: G. Mayer, N. Blanchemain, C. Dupas-Bruzek, M. Traisnel, D. Derozier, L.D. Laude, H.F. Hildebrand
Abstract: LASER Excimer irradiation can modify surface properties for biocompatibility improvement of a medical device. The PETs from 3 different origins were used in this study. The samples have been irradiated by excimer LASER with 10 different energies. The surface profile, the surface energy and the materials crystallinity have been assessed. Biological characterizations were made with human embryonic epithelial cells L132: proliferation, vitality, viability, adhesion with the p-NPP, and morphology. - The profile measurements allowed to establish the ablation threshold, which was 36 mJ/cm². The surface hydrophilic state increased reciprocally with the irradiation intensity. The gain is 13 %. The irradiated and non-irradiated product has identical cristallinity. PET was shown not to be toxic for L132 cells. Cell proliferation and cell vitality showed dose-dependant increases reciprocal to the irradiation energy (from 88 to 138% with respect to control). The correlation was highly significant (R² = 0,8). SEM micrographs show that the cells are better spread on the surface of irradiated PET than on untreated PET.
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