Papers by Author: Jin Wang

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Authors: Jia Ju Tang, Jin Wang, Chang Jiang Pan, Ya Jun Weng, Nan Huang
Abstract: Three kinds of curcumin-loaded films (3wt%, 5wt%, 8wt%) were prepared using poly(lactic acid-co-glycol acid (PLGA) as the carrier of curcumin, and studied. The result of Fourier transform infrared spectroscopy (FTIR) and X-ray electron spectroscopy (XPS) show that the curcumin is dispersed in the PLGA films. High performance liquid chromatography (HPLC) analysis suggests that the release of curcumin can last 22-43 days. A fewer number of adhered and activated platelets are observed on the curcumin-loaded PLGA films. The activated partial thromboplastin time (APTT) increases for all curcumin-loaded films.
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Authors: Ling Ren, Jin Wang, Jia Ju Tang, Ch.J. Pang, M. Maitz, Nan Huang
Abstract: Sirolimus-loaded poly (lactic acid-co-glycol acid) (PLGA) films were prepared by a casting method. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to determine the optimal concentration of sirolimus for vascular smooth muscle cell (VSMC) inhibition effect. Based on the results of MTT, three samples of different weight percent loaded-sirolimus (2wt%, 6wt%, 15wt%) PLGA films were selected. The release of lactate dehydrogenase (LDH) was used to evaluate the immediate toxicity of the sirolimus-loaded PLGA films and it was shown that the three different concentrations of sirolimus-loaded films have low toxicity. Alamar blue results indicate that the sirolimus-loaded films have better antiproliferation effect than 316 stainless steel (SS). The higher the sirolimus concentration in the film is, the better is the antiproliferation effect. These films may be used for stent coating to inhibit in-stent restenosis induced by VSMC proliferation.
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Authors: Jin Wang, P. Li, H. Sun, Ping Yang, Y.X. Leng, Ji Yong Chen, Nan Huang
Abstract: We report a study involving chitosan chains immobilized on poly (ethylene terephthalate) (PET) films by plasma and ultraviolet (UV) grafting modification. The surface structure of the modified PET is determined by means of attenuated total reflection Fourier transform infraed spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS). The results show that the chains of chitosan are successfully grafted on the surface of PET. Platelet adhesion evaluation in vitro is conducted to examine the blood compatibility in vitro. Scanning electron microscopy (SEM) and optical microscopy reveal that the amounts of adhered, aggregated and morphologically changed platelets are reduced on the chitosan-immobilized PET films. The number of platelet adhered on the modified film is reduced by almost 48% compared to the amount of platelets on the untreated film. Our result thus shows that chitosan immobilized on the PET surface improves blood compatibility.
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Authors: Ya Jun Weng, Ruixia Hou, Dong Xie, Jin Wang, Nan Huang
Abstract: Heparin is covalently immobilized onto the surface of anatase TiO2 film using the bifunctional linking reagent, APTES (3-Aminopropyltriethoxylsilane), which can be bonded to the film by reaction between ethoxyl of the linking reagent and hydroxyl of the film. Compared to the control, the immobilization is enhanced by phosphoric acid chemical pre-adsorption on the film for there is more hydroxyl group present. Platelets deposited to heparinized surface showed only minor spreading and aggregation. The results of this study suggest that heparin immobilization to anatase TiO2 films via phosphoric acid interface may improve the in vivo blood compatibility.
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Authors: Lan Xin Xu, Peng Li, Jin Wang, Yong Xiang Leng, Hong Sun, Guang Jun Cai, Nan Huang
Abstract: In order to improve the abrasion property of polyoxymethylene (POM),diamond like carbon (DLC) film was deposited on POM substrate by the method of magnetic filtered cathodic vacuum arc deposition (MFCVAD). X-ray photoelectron spectroscopy (XPS) showed that the sp3 fraction decreased with the negative bias voltages increased. According to the result of the nano-indenter, the hardness of POM plates coated with DLC films was significantly harder than POM substrate. The results of ball-on-disc tribological testing demonstrated that the hardest DLC film had the lowest friction coefficient (0.11). The evaluation of in vitro platelet adhesion test indicates that the numbers of adhered and activated platelet on the DLC film deposited under the condition of 0V and 9min were remarkably decreased compared with that of the untreated POM.
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Authors: Jin Wang, Jia Ju Tang, Ling Ren, Chang Jiang Pan, Nan Huang
Abstract: Rapamycin/curcumin co-loaded and rapamycin-loaded poly(lactide-co-glycolide)(PLGA) films were prepared by a casting method. The components of different drug-loaded films were analyzed by Fourier transform infrared spectroscopy (FTIR), and the major peaks of rapacymin and curcumin were both observed in these films. Compared to the rapamycin-loaded film, the results of in vitro platelet adhesion tests show that the number of adhered platelets reduce, and few aggregated and activated platelets are observed. The APTTs (activated partial thromboplastin time) of rapamycin/curcumin co-loaded films is nearly 6 seconds longer than for the rapamycin-loaded PLGA films. All results indicate that curcumin may suppress thrombosis activated by rapamycinloaded film, and improve the anticoagulative property. The results of alamar blue indicate that the rapamycin/curcumin co-loaded film has better antiproliferation effect than 316 stainless steel (SS).
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Authors: Bo Feng, Jie Weng, Yu Liang, Shu Xin Qu, Jin Wang, Xiong Lu
Abstract: A network-like porous layer on titanium was prepared by acid-etching with a mixture of CaCl2 and HCl. The pores ranged from 1 to 10!m. The small pores distributed in big pores. In the simulated biological environment, porous octacalcium phosphate (OCP) coatings spontaneously formed on the porous- surfaced titanium. Pre-calcification after acid-etching accelerated OCP precipitation. The OCP coatings had big pores of about 25!m and small pores of 1~3!m, the latter distributed in the former at different depth.
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Authors: Chang Jiang Pan, Jin Wang, H. Sun, Nan Huang
Abstract: In this paper, polyethylene glycol (PEG) of various different molecular weights was grafted onto PET films using plasma surface grafting modification. The surface structure of PEG-grafted PET films was analyzed by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS), suggesting that the surface structure and composition of PET films changes due to the presence of PEG. Blood compatibility was characterized by in vitro platelet adhesion experiments and coagulation factors. The tests of platelet adhesion and coagulation factors in vitro suggest that PEG grafted onto polymer surfaces can improve the blood compatibility of PET films remarkably. The modified PET films were pre-coated with albumin and fibrinogen respectively; platelet adhesion tests in vitro then indicated that samples pre-coated with albumin have better blood compatibility than with fibrinogen, resulting in the conclusion that the albumin can improve blood compatibility. The contact angle of PEG-grafted films was measured by the sessile drop method and the surface free energy and interface free energy were induced. It is indicated that the PEG-grafted PET films have the characteristic of preferentially adsorbing albumin.
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Authors: Nan Huang, Ping Yang, Yong Xiang Leng, Jun Ying Chen, Jin Wang, Guo Jiang Wan, Hong Sun, X. Wu, An Sha Zhao
Abstract: This paper presents recent activities on the surface modification of blood contacting biomaterials and devices in the author’s laboratory. Surface coating of inorganic films on materials for artificial heart valves, ventricular pumps and coronary stents, such as titanium, stainless steel and low temperature isotropic pryolitc carbon, etc, shows a significant improvement in the anticoagulation behavior. Further, the formation of functional groups such as hydroxyl or amino groups and the binding of biomolecules as well as seeding of endothelial cell shows the promise of biomimetic surface formation. Plasma grafting on materials for artificial heart valve sewing cuff, extracorporeal circulation tube, etc, such as PET, PU, PVC polymers, revealed a significant improvement of anti-platelet adhesion as well as anti-bacterial properties.
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Authors: An Sha Zhao, Ping Yang, Yong Xiang Leng, Jun Ying Chen, Jin Wang, Guo Jiang Wan, Nan Huang
Abstract: Ti-O film is a kind of potential biomaterial may be applied in medical devices. But the mechanism of its good biocompatility is not so clear. This study revealed that when Titanium oxide contact with macrophage and plasma, the activation, adhesion and secretion of inflammatory molecule MCP-1 of macrophage is lower than reference material. Ti-O film also show minor contact activation to plasma. So reducing the host reaction including contact activation and inflammation may be the important reason for the good biocompatibility of Ti-O film.
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