Papers by Author: Suwannee Panomsuk

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Authors: Tittaya Suksamran, Theerasak Rojanarata, Tanasait Ngawhirunpat, Suwannee Panomsuk, Praneet Opanasopit
Abstract: The aim of this study was to prepare Caalginate and chitosan (CS)Caalginate microparticles for peroral delivery of ovalbumin (OVA). Microparticles containing different loading of OVA (10, 20 and 40 % w/w) were prepared by cross-linking alginate with calcium chloride using an electrohydrodynamic spraying technique, and then coated with CS. The particle sizes of OVA-loaded microparticles were in the range of 1-5 µm. The negative charge was obtained for Caalginate microparticles (-14±1.9 mV) whereas CSCaalginate microparticles were positive charge (+6.06±3.4 mV). Caalginate microparticles with initial 20% w/w OVA showed the highest entrapment efficiency and amount of OVA content (24.91±0.4% and 33.22±0.1 mg/g, respectively) as similar to CSCaalginate microparticles with initial 20% w/w OVA that showed the highest entrapment efficiency and amount of OVA content (35.74±0.1% and 10.35±0.5 mg/g, respectively). It was found that the release rate of OVA from Caalginate microparticles was higher than CSCaalginate microparticles, and the lowest release rate, sustained release for 24 h, was found in the initial 40% w/w OVA. This study revealed that CSCaalginate microparticles have a considerable potential as controlled release antigen delivery systems.
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Authors: Nanthida Wonglertnirant, S. Tipwichai, Praneet Opanasopit, Theerasak Rojanarata, Suwannee Panomsuk, Tanasait Ngawhirunpat
Abstract: Ketoprofen transdermal patches (KTPs) were fabricated using an acrylic pressure sensitive adhesive (PSA) polymer. The influence of different factors (amount of PSA, drug content, and pressure applying on the backing membrane during preparation) on the characteristics of ketoprofen patch (thickness, W/A ratio, and adhesiveness of matrix film) and in vitro drug release behavior were investigated. The results revealed the successful fabrication and a good physical appearance of KTPs using acrylic PSA. Microscopic observations, FTIR spectra, and DSC thermograms were permitted to demonstrate that the drug was dispersed molecularly in the polymer. As the amount of PSA in the adhesive matrix was increased, the release rate of ketoprofen was decreased. Contrarily, the drug release rate was increased corresponding to the increase of ketoprofen content in the adhesive matrix. There was no significant difference in the release rate when the pressure applying on the backing membrane was varied. The kinetic of ketoprofen release from acrylic matrix type transdermal patches followed the Higuchis diffusion model.
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Authors: Tanasait Ngawhirunpat, Theerasak Rojanarata, Suwannee Panomsuk, Praneet Opanasopit
Abstract: The aim of this study was to prepare and characterize electrospun polyvinyl alcohol (PVA) nanofiber mats loaded with capsaicin (CC) as a transdermal drug delivery system. The amount of CC loaded in the base PVA solution (10 %w/v solution) was 0.025, 0.0375 and 0.05 %, based on the dry weight of PVA (% wt). The average diameters of these fibers ranged from 121 to 165 nm. In all concentrations of CC loaded in spun PVA fiber mats, an amorphous nanodispersion of CC with PVA was obtained. The tensile strength of the as-spun fiber mats was lower than that of the as-cast PVA films. The release rate of CC from CC-loaded as-spun PVA was significantly higher than from CC-loaded as-cast PVA films, and increased when the CC content in both CC-loaded as-spun PVA and CC-loaded as-cast PVA films increased. Our research suggests a potential use for CC-loaded electrospun PVA mats as a transdermal drug delivery system.
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Authors: Ponwanit Jarenputtakrun, Praneet Opanasopit, Suwannee Panomsuk, Tanasait Ngawhirunpat
Abstract: The aim of this study was to prepare and investigate the isosorbide dinitrate transdermal patches (IDPs) in the concentration of 40 mg/cm2. Acrylic pressure sensitive adhesives (PSA) were used to formulate IDPs. IDPs were prepared by casting method. The effect of content of PSA, and concentration of enhancer, propylene glycol, in the formulations were evaluated. IDPs were investigated for their thickness, weight/area ratio, adhesiveness and in vitro skin permeation. The higher the content of PSA in the formulation, the higher the thickness and the W/A ratio. Propylene glycol added in the formulation (2.5, 5, 10%) significantly enhanced the skin permeation of ISDN. The higher the content of PG, the higher the flux of ISDN through the skin. Our research suggests that isosorbide dinitrate loaded with 10% of propylene glycol in acrylic matrix pressure sensitive adhesive can be potentially used as a transdermal drug delivery system.
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Authors: A. Apirakaramwong, Perayot Pamonsinlapatham, S. Techaarpornkul, Praneet Opanasopit, Suwannee Panomsuk, S. Soksawatmaekhin
Abstract: Chitosan (CS) has a high potential for gene delivery into mammalian cells. However, its uptake mechanism is not well clarified. We investigated the effects of inhibitors of clathrin-mediated endocytosis (chlorpromazine), caveolae-mediated endocytosis (genistein), macropinocytosis (LY 29004 and wortmannin), microtubuli polymerization (nocodazole) and of membrane cholesterol recycle (methyl-β-cyclodextrin) on the transfection efficiency with CS/pEGFP complexes and on the internalization of CS/rhodamine-labeled pEGFP complexes by hepatoma cell line (Huh 7 cells). The transfection was blocked by nocodazole, genistein, and methyl-β-cyclodextrin, respectively. CS/DNA complexes internalization was clearly inhibited by genistein. We conclude that the complexes uptake predominantly by caveolin-mediated pathways. In addition, fluorescence colocalization studies with acidotropic probes, LysoSensor dye, illustrated that CS/DNA complexes are targeted to lysosomes for the degradation after internalization.
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Authors: Suwannee Panomsuk, S. Indis, P. Panyasaroj, S. Chootrakulwattana, Praneet Opanasopit, Theerasak Rojanarata
Abstract: Centella asiatica (4% w/w) oral bases were formulated in order to treat ulcers, both in the oral cavity and topical area. Two types of bases, oleaginous and aqueous, were used. The oral bases were evaluated for their physical appearances, pH, viscosity, physical stability and adhesion. PEG and Carbopol based exhibited suitable properties. The viscosity and adhesion of some bases were enhanced by adding dry powder polymer. The amount of asiaticoside, active ingredient, in the extract and in the formulations was analyzed by HPLC.
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