Papers by Author: Yasuhito Tanaka

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Authors: Yasuhito Tanaka, Yoshinori Takakura, K. Kadono, Akira Taniguchi, K. Hayashi, Jin Iida, Koh Ichi Sugimoto, Yasuaki Tohma, Hajime Ohgushi
Authors: T. Henkel, Yasuhito Tanaka, Naoto Kobayashi, Shin Ichi Nishizawa, Shunichi Hishita
Authors: Hajime Ohgushi, Shigeyuki Kitamura, Noriko Kotobuki, Motohiro Hirose, Hiroko Machida, Akira Oshima, Yasuhito Tanaka, Yoshinori Takakura
Abstract: Alumina ceramics have excellent mechanical and biocompatible properties, but are bioinert and hence have no bone-bonding properties. We took a tissue engineering approach in an attempt to modify the ceramic surface and so provide an osteogenic/osteoconductive milieu. We used fresh human bone marrow cells obtained from the iliac crest by needle aspiration for culture expansion of mesenchymal stem cells (MSC) followed by in vitro osteogenic differentiation on both tissue culture polystyrene (TCPS) and alumina ceramics. We have succeeded in expanding the number of MSC from all 35 cases and compared the differentiation capability of selected MSC on alumina ceramics to that on TCPS. The cells on both substrata showed extensive alkaline phosphatase staining and mineralization as evidenced by calcein uptake. Biochemical analyses revealed high levels of alkaline phosphatase activity, osteocalcin expression, and calcium content. These data indicate that an alumina ceramic surface can support a differentiation cascade of MSC resulting in osteoblastic phenotype expression of the cells. Based on these results, we have performed clinical applications of tissue engineered total joint replacements for osteoarthritic patients.
Authors: Yoshinori Kobayashi, Yasuhito Tanaka, H. Goto, Kei Matsuoka, Yoshinobu Motohashi
Authors: Ludwik Dobrzyński, Yasuhito Tanaka, Yoji Sakurai
Authors: Hajime Ohgushi, Hiroko Machida, M. Ikeuchi, Tetsuya Tateishi, Yasuaki Tohma, Yasuhito Tanaka, Yoshinori Takakura
Authors: Yasuhito Tanaka, Hajime Ohgushi, Shigeyuki Kitamura, Akira Taniguchi, Koji Hayashi, Shinji Isomoto, Yasuaki Tohma, Yoshinori Takakura
Authors: Hajime Ohgushi, Hiroko Machida, Akira Oshima, Noriko Kotobuki, Motohiro Hirose, Akira Fujisawa, Yasuhito Tanaka, Yoshinori Takakura
Abstract: After culture expansion of mesenchymal stem cells (MSCs) from a few milliliter of fresh patient’s bone marrow, we applied the MSCs on alumina ceramic ankle prosthesis and further cultured in an osteogenic medium for 2 weeks. After the culture, the MSCs differentiated into osteoblasts, which fabricated bone matrix on the surface of ceramic prosthesis. The expansion of MSCs followed by osteogenic differentiation was done using the commercially available medium with some chemicals and patient’s own serum. The MSCs well proliferated and differentiated into osteoblasts, even the MSCs were from old aged (more than 70 years old) patients. The tissue engineered ceramic prostheses were implanted into osteoarthritic patients. Typical X-ray findings showed that radiodense areas began to appear around the cell-seeded areas on the prosthesis about 2 to 3 months after the operation. These findings confirmed the importance of tissue engineering approach for early bone fixation and the approach can be done using small number of bone marrow cells and patient’s own serum without adding animal-derived products.
Authors: Junji Saida, K. Ohsaki, Yasuhito Tanaka, M. Uda, Kenji Okazaki
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