Papers by Author: Ying Jun Wang

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Authors: Chun Lin Deng, Ying Jun Wang, Yao Wu, Xin Long Wang, Xiao Feng Chen, Hua De Zheng, Ji Yong Chen, Xing Dong Zhang
Abstract: Porous HA/TCP bioceramics were immersed in pure dog serum to observe apatite formation. Deposited crystals were examined using SEM. Results showed that beamed sheet-like crystals formed on the surface of ceramics granules, and after postponement immersion time, crystals extended and became bigger. EDS and IR results suggested formed crystals were defect-calcium type carbonated hydroxyapatite. HRTEM photograph suggested formation process of new-formed crystals from non-crystal to crystal in serum. Directional organisms acted maybe as a template in process of crystals formation, so new crystals developed along certain direction.
Authors: Chun Lin Deng, Ying Jun Wang, Yao Wu, Ying De Qin, Hong Song Fan, Ji Yong Chen, Xing Dong Zhang
Abstract: Dense HA/TCP bioceramics were immersed in pure bovine serum, rabbit serum and dog serum to observe apatite formation. Deposited crystals were examined using SEM. Results showed that some needle-like crystals formed on surface of sterilized HA/TCP, and needle crystals developed into sheet crystals and stick crystals after ceramics were immersed in bovine serum and rabbit serum respectively. The growth of crystals was maybe affected by the content of calcium, various kinds of albumen and alkaline phosphatase in different serums and the different pH of serum.
Authors: Gang Wu, Ying Jun Wang, Hua Ding Lu, Xiao Feng Chen, Jian Dong Ye, Cheng Yun Ning, Na Ru Zhao
Abstract: In this article, a multilayer tissue engineering scaffold has been fabricated. The uppermost layer is consisted by the collagen and the downmost layer is consisted by the collagen/hydroxyapatide. Between the two layers, there have several continues changed collagen/HA layers at different ratio. These gradient scaffolds have been made by the freeze dried method. The morphology of the multiphase scaffold has been observed by the SEM. The chondrocytes from New Zealand rabbit knee joint were separated, harvested and cultured on the top layer of the scaffold. The histological and the immunohistochemical testing show that the chondrocytes keep its normal type in the 2 culture weeks.
Authors: Gang Wu, Ying Jun Wang, Kun Wei
Abstract: The bioactivity of HA has been tested by its osteoconduction ability in vivo. Nano HA has higher specific surface area and was presumed to have high bioactivity. In this paper, nano needle HA was synthesized by the microemulsion method. The properties of such nano materials were analyzed by the XRD and TEM. The impact of the nano HA on the expression of two hBMSCs’ genes, inhibitor differentiation 2 and ostopontin, and the ALP activity was researched in this paper.
Authors: Yu Dong Zheng, Ying Jun Wang, Xiao Feng Chen, Hua Ding Lu, Qing Liu
Abstract: In this work, a new kind of bioactive PVA/HA hydrogels use as artificial cartilage were prepared by in situ sol-gel synthesis method. Simulated experiments were performed to evaluate and compare the bioactive ability and properties of the hydrogels in simulated body solution. PVA/HA Cartilage implants were developed and used to repair articular cartilage defect in rabbit knee. The macroscopic and histological observation of animal experiments displayed that the implanted hydrogels combined tightly to ambient tissues, and some bone-like tissue grew into the bottom of the implants from the base-bone to form more deep-set binding.
Authors: Xiao Feng Chen, Ying Jun Wang, Chun Rong Yang, Na Ru Zhao
Abstract: The bone tissue engineering scaffold was developed by compounded the type I collagen with the porous scaffold of the sol-gel derived bioactive glass (BG) in the system CaO-P2O5-SiO2. The resultant porous scaffold was treated in supersaturated calcification solution (SCS) to form the surface layer of hydroxyl-carbonate-apatite (HCA) since the type I collagen possessed good biocompatibility and bio-absorbability, and also, the ability of inducting calcium phosphates to precipitated inside and outside the collagen fibers where the collagen fibers acted as bio-macromolecules template for formation of bone-like inorganic minerals in nature bone such as: octo-calcium phosphate (OCP), tri-calcium phosphate (TCP) and hydroxyl-carbonate-apatite (HCA). On the other hand, the sol-gel derived bioactive glass also played an important role in formation of the above bio-minerals owing to its serial chemical reactions with the body fluid. The in vitro study in supersaturated calcification solution SCS indicated that the surface of the porous scaffold was able to induce formation of bone-like HCA crystals on the pore walls of the scaffold which possessed satisfactory cells biocompatibility.
Authors: Ying Jun Wang, Hong Xu, Li Ren, Yu Dong Zheng, Xiao Feng Chen
Abstract: In order to form the firm active fixation with the adjacent bone, a new kind of bioactive composite hydrogel was prepared as calcic layer of cartilage with polyvinyl alcohol (PVA) and bioglass (BG). The biomineralization properties of the PVA/BG biocomposite hydrogel were studied through Fourier transformed infrared spectroscopy, XRD and SEM with EDX. Results showed that hydroxylcarbonateapatite was formed on the surface of the PVA/BG biocomposite hydrogel after the biocomposite hydrogel soaked into SBF solution. The composite possesses good mineralization properties and could form good firm active fixation with the adjacent bone.
Authors: Ying Jun Wang, Chun Rong Yang, Hua De Zheng, Xiao Feng Chen, Cheng Yun Ning, Li Ren, Chun Lin Deng
Abstract: The PCL plates hydrolyzed by NaOH aqueous solutions and carboxylate groups were introduced onto the surfaces of specimen. Specimens were treated by CaCl2 and K2HPO4⋅3H2O under the normal-pressure condition and low-pressure of 103 Pa condition for 30min separately. Dense and uniform bone-like layers could be formed on the surface of specimens after mineralizing for less than 24h in simulated body fluids (SBF). The low-pressure condition could accelerate the formation of apatite layer.
Authors: Huan Wen Ding, Qiang Tu, Hong Wang, Suo Lin Zeng, Bao Liu, Jian Jian Shen, Can Yi, Ying Jun Wang, Qing Shui Yin
Abstract: Computed tomography is used in 13 clinic cases with bone malignant tumor to get 2-dimensional data of diseased area. Reconstruction of 3- dimensional anatomical model, excision range of bone, designing of individual surgical template and bone prosthesis was accomplished preoperatively by computers. Based on computer-assisted-design (CAD) proposal, we cut the tumor accurately, and use allografts bone with personalized prosthesis to reconstruct the bone defect after tumor excision. It is concluded that we can design excision range of bone easily, cut them accurately, reconstruct bone defect of diseased area precisely with the CAD technology.
Authors: Jing Di Chen, Ying Jun Wang, Xiao Feng Chen
Abstract: Owing to the unique characters of the hybrid scaffold components, a novel biodegradable porous composite scaffold was prepared. The nano hydroxyapatite (HAP) (73~136 nm) was crystallized in situ on the organic polyelectrolyte complex matrix through a biomimetic method. The polyelectrolyte complex composed of chitosan and hyaluronic acid had strong impacts on the formation of the nano HAP and directed in situ crystallization of the nano HAP as template. The in situ nano HAP reduced the interfacial energy and presented nano intensifier to the nano hybrid scaffold. Meanwhile, the scaffold kept high porosity.
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