Papers by Author: Hee Seung Bom

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Authors: Mi Kyong Yoo, You Kyoung Kim, Hwan Jeong Jeong, Hee Seung Bom, Chong Su Cho
Abstract: To improve the specific accumulation in tumor sites and aqueous solubility of atRA, the core-shell type of folate-PEG-g-PEI/atRA nanoparticles were prepared by complexation between cationic PEI segments in the copolymers and anionic charged atRA, and then characterized by 1HNMR, ELS, XRD, and TEM. In vitro atRA release from the nanoparticles was investigated as a function of drug content in sink condition. Cytotocicity of atRA against HepG2, KB cell lines were also evaluated by MTT assay. The lower the drug content, the faster atRA release. atRA incorporated in folate-PEG-g-PEI/atRA nanoparticles showed much higher cytotoxic effect compared with atRA itself.
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Authors: Tae Hee Kim, In Kyu Park, Su Il Kim, Hwan Jeong Jeong, Hee Seung Bom, Chong Su Cho
Abstract: Chitosan has been considered to be a good candidate for gene delivery system, since it is already known as a biocompatible, biodegradable, and low toxic material with high cationic potential. However, low specificity and low transfection efficiency of chitosan need to be overcome prior to clinical trial. In this review paper, chemical modification of chitosan for enhancement of cell specificity and transfection efficiency was explained. Also, chemical modification of chitosan for the stability of chitosan/DNA complexes was reviewed.
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Authors: You Kyoung Kim, In Kyu Park, Hu Lin Jiang, Rohidas B. Arote, Hwan Jeong Jeong, Eun Mi Kim, Myung Haing Cho, Hee Seung Bom, Chong Su Cho
Abstract: Polypropylenimine (PPI) dendrimers have been used by many researchers as gene delivery carriers due to their high functionality. Glucose as a kind of carbohydrate is biocompatible and hydrophilic. In this study, we synthesized glucosylated PPI (G-PPI) dendrimers to reduce cytotoxicity. Glucose substitution of G-PPI dendrimers was determined by the sulfuric acid micromethod. The G-PPI dendrimer was complexed with plasmid DNA in various N/P ratios, and the complex was characterized. G-PPI dendrimers showed good DNA binding ability and high protection of DNA from nuclease attack. The G-PPI dendrimer had low cytotoxicity compared to PPI dendrimer by cytotoxicity assay. Also, transfection efficiency was influenced by glucosylation degree and the transfection efficiency for the G-PPI-5 was slightly higher than that of PPI dendrimer in HeLa cell line.
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