Papers by Author: Masataka Sakane

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Authors: Shinobu Tanaka, Masataka Sakane, M. Tanaka, Isamu Yamaguchi, Hitoshi Shimojo, Kenji Kato, Tetsuya Tateishi, Yutaka Miyanaga
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Authors: Yasutaka Yamada, Atsuo Ito, Hiroko Kojima, Masataka Sakane, Shumpei Miyakawa, Toshimasa Uemura, Racquel Z. LeGeros
Abstract: Osteoclasts isolated from rabbits were cultured on zinc-containing tricalcium phosphate (ZnTCP) disks with zinc contents of 0.316 and 0.633 wt%, and on β-tricalcium phosphate (TCP) disks with nearly identical porosities, grain sizes and surface roughnesses. ZnTCPs directly suppressed the resorption activity of mature osteoclasts by enhancing apoptosis. We hypothesized that resorbing osteoclasts attached to ZnTCP locally accumulate zinc ions within the space defined by the clear zone during resorption, which in turn leads to apoptosis induction, even though the change in chemical composition of the culture medium is very small.
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Authors: Masashi Iwasashi, Masataka Sakane, Yasushi Suetsugu, Naoyuki Ochiai
Abstract: Unidirectional porous hydroxyapatite (UDPHAp) was developed which has microstructure in that cross sectionally oval pores 100 ~ 300µm in diameter penetrate through the material, and that is suitable for osteogenesis and angiogenesis.The porosity of the UDPHAp was 75 % and the compression strength was 14 MPa. A cortical bone defect was made at proximal tibia of Japanese white rabbit, and a trapezoidal prisms shaped UDPHAp was implanted. By histlogical evaluation, 2 weeks after implantation, new bone and new capillary was observed inside UDPHAp. Twelve weeks after implantation, new bone formation was observed in 41.6 % of the porous area. The results of this study suggest a great possibility of utilizing it in actual clinical setting as a bone substitution.
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Authors: Shinobu Kobayashi, Masataka Sakane, Hirotaka Mutsuzaki, Hiromi Nakajima, M. Tanaka, Yutaka Miyanaga, Naoyuki Ochiai
Abstract: We hybridized calcium phosphate (CaP) with human semitendinosus and gracilis (ST/G) tendon grafts using an alternate soaking process. To evaluate quantitatively and histologically assess the CaP hybridized human ST/G tendon grafts, we classified them into three groups according to their soaking time – number of soaking cycle: 30 sec – 20 cycles (Group A), 1 min – 15 cycles (Group B), 3 min – 5 cycles (Group C). The tendon grafts were divided into three parts: tibial end (TE), femoral end (FE) and intra-articular (IA) portion. TE was secured using the Krackow technique with No. 2 nonabsorbable sutures, and an Endobutton-CL (Smith & Nephew, USA) was passed through the looped FE, as performed clinically. Then, the IA portion was covered with the sleeve of a rubber glove to prevent CaP hybridization. More soaking cycles induced greater deposition of CaP in the tendon grafts when the total soaking time was the same. Covering the IA portion with a rubber sleeve prevented of CaP deposition. A large amount of CaP in TE was deposited because suture holes increased the total contact area with the solutions.
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Authors: Hirotaka Mutsuzaki, Masataka Sakane, Yumi Katayoshi, Sinya Hattori, Hiromi Nakajima, M. Tanaka, Naoyuki Ochiai
Abstract: Calcium phosphate (CaP) hybridized to a whole tendon graft delayed cell repopulation in anterior cruciate ligament (ACL) reconstruction in rabbits. However, a tendon graft masked with an adhesive tape at the intra-articular (IA) portion to prevent CaP hybridization did not delay cell repopulation. Synovial tissues can adhere to the tendon graft and can invade the tendon graft masked at the IA portion. The masking induced an effect similar to that of the unhybridized tendon graft. The CaP hybridized tendon grafts masked at the IA portion showed cell repopulation 2 weeks earlier than the unmasked CaP hybridized tendon grafts.
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Authors: Tetsushi Taguchi, H. Saito, Masashi Iwasashi, Masataka Sakane, S. Kakinoki, Naoyuki Ochiai, Tetsuya Tateishi
Abstract: A biocompatible glue consisting of human serum albumin (HSA) and citric acid derivative (CAD), named CAD-A glue was developed. CAD was successfully synthesized by the reaction between citric acid and N-hydroxysuccinimide in the presence of 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride. When the CAD-A glue was applied to the collagenbased casings, it took 7 min to obtain half of maximum bonding strength (760 g/cm2). The bonding strength of this glue to collagen-based casings increased with increasing of HSA concentration.The bonding strength of CAD-A glue increased with increasing CAD concentration up to 200 mM, and then decreased with increasing CAD concentration under the fixed HSA concentration (50 w/w%). The CAD-A glue showed excellent wound closure ability rather than fibrin glue when applied to the mouse skin. These results suggested that this developed glue had both tissue compatibility and bonding strength for use in clinical field.
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Authors: Yu Sogo, Atsuo Ito, Hirotaka Mutsuzaki, Ayako Oyane, M. Onoguchi, Noboru Ichinose, Masataka Sakane, Naoyuki Ochiai
Abstract: A FGF-2-apatite composite layer (FGF-AP layer) was formed on the surface of Ti screws in a supersaturated calcium phosphate solution supplemented with FGF-2. By an in vitro study using fibroblastic NIH3T3 cells, it was confirmed that FGF-2 was immobilized in the layer without complete denaturation although the composite layer was formed at 37°C. When Ti screws with the FGF-AP layer were percutaneously implanted in the proximal tibial metaphysis of 16 rabbits, no osteomyelitis was observed in any rabbits although a FGF-2-free AP layer allowed osteomyelitis in some cases in our previous study. These results suggest that a FGF-AP layer formed on Ti screws is useful for resisting bacterial infection during external fixations.
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Authors: Toru Funayama, Hiroshi Noguchi, Toshinori Tsukanishi, Masataka Sakane
Abstract: To evaluate the osteoconductive potential of connected porous hydroxyapatite (HAp), we histologically analyzed the newly formed bone inside unidirectional porous HAp (Regenos®, Kuraray, Japan; 75% porosity, n=17) and interconnected porous HAp (Neobone®, Covalent Materials, Japan; 75% porosity, n=10) 26 weeks after their implantation as bone spacers between the split lumbar laminae of goats. As a control, non-connected porous HAp spacers (Apaceram®, Pentax, Japan; 50% porosity, n=5) were used. After staining non-decalcified samples with Villanueva Goldner, changes in pore shape were evaluated microscopically and new bone formation in HAp spacers was quantitatively analyzed. In addition, blood vessel distribution was evaluated by hematoxylin and eosin staining. Changes in pore shape were observed in 76% of the Regenos® spacers and 90% of the Neobone® spacers but were not detected in the Apaceram® spacers. Only limited new bone formation was observed in the Regenos® and Neobone® spacers, whereas vascular-like structures were detected in 82% of the Regenos®, 70% of the Neobone®, and 80% of the Apaceram® spacers. The changes in pore shape were thought to have resulted from the low initial compression strength of the connected porous HAp, which may have limited the inherent osteoconductive potential of connected HAp. Our findings suggest that the maintenance of pore shape is required for promoting new bone formation in connected porous HAp when used as lamina spacers in spinal surgery.
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Authors: Tetsuya Abe, Masataka Sakane, Toshiyuki Ikoma, Toru Tonegawa, Tomonori Yoshioka, M. Tanaka, Naoyuki Ochiai
Abstract: The extent of osseous involvement, particularly spinal cord compression, is directly correlated with patient survival. To treat metastatic spine cancer, we have developed novel paclitaxel-loaded hydroxyapatite-alginate gels. In this study, an intraosseous spinal cancer model in rats was used to investigate the efficacy of local treatment. Ten rats were randomized into two groups, a local treatment group and a control group. Disease-free time and survival rate in the local treatment group were significantly longer in this model. (p<0.05)
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Authors: Tomonori Yoshioka, Toshiyuki Ikoma, Akira Monkawa, Shunji Yunoki, Tetsuya Abe, Masataka Sakane, M. Tanaka
Abstract: Hydroxyapatite (HAp)-alginate gels were developed as drug delivery carriers of the anti-cancer drug, water-insoluble paclitaxel (Taxol). The spray-drying technique was employed for loading the paclitaxel into spherical HAp microparticles with 1 to 20 μm in diameter. The microparticles loaded with 2.4 or 7.3wt% of paclitaxel were then mixed with sodium alginate, which was followed by Ca2+-mediated gelation. The compressive strength of the HAp-alginate gels and the release of paclitaxel from the gels in a medium were investigated in vitro. The alginate matrix was effective for the achievement of controlled release of anti-cancer drugs.
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