Papers by Author: Tetsuya Tateishi

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Authors: Guo Ping Chen, Takashi Ushida, Tetsuya Tateishi
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Authors: Guo Ping Chen, Daisuke Akahane, Naoki Kawazoe, Yoshio Shirasaki, M. Tanaka, Katsuyuki Yamamoto, Tetsuya Tateishi
Abstract: A novel collagen sponge that can protect cell leakage during cell seeding was developed by wrapping all the surfaces except the upside of a collagen sponge with membrane that has pores smaller than cell. The collagen sponge was used for three-dimensional culture of human bone marrow-derived mesenchymal stem cells (MSCs). The cells adhered to the collagen, and proliferated to fill the spaces in the sponge. The cell seeding efficiency was higher than 95%. The MSCs cultured in the collagen sponge in the chondrogenic induction medium supplemented with TGF-β3 and BMP6 expressed genes encoding type II collagen, SOX9 and aggrecan. HE staining indicated the round morphology of differentiated cells and the extracelluler matrices were positively stained by safranin O and toluidine blue. Type II collagen and cartilage proteoglycan were detected by immunostaining with anti-type II collagen and anti-cartilage proteoglycan. These results suggest the chondrogenic differentiation of MSCs. The collagen sponge facilitated cell seeding and chondrogenic differentiation of MSCs, and will be useful for cartilage tissue engineering.
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Authors: Takashi Ushida, Tamotsu Tamaki, Guo Ping Chen, Yoshikazu Umezu, Tetsuya Tateishi
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Authors: Toshimasa Uemura, J. Dong, Takafumi Yoshikawa, Y. Hakamatsuka, H. Irie, Hisahiro Inoue, Tetsuya Tateishi
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Authors: Shinobu Tanaka, Masataka Sakane, M. Tanaka, Isamu Yamaguchi, Hitoshi Shimojo, Kenji Kato, Tetsuya Tateishi, Yutaka Miyanaga
479
Authors: Yu Jiang Fan, Guo Ping Chen, M. Tanaka, Tetsuya Tateishi
Abstract: Polyamides containing amino acid residues were synthesized through a chemoenzymatic route. At first, monomers containing amino acid esters at both ends were prepared from amino acid esters and bifunctional acid chlorides through a Scotten-Baumann reaction under organic condition. Diamine was used as bifunctional nucleophilic reagent to aminolysis the amino acid ester bonds of these monomers under protease catalysts, leading to the polycondensation to form the desired polyamides containing amino acid residues. Spectra characterizations confirmed the structure of obtained polyamides. The factors that influence the polymerization, including the type of enzymes, the amount of enzymes, and the water contents in the solvent, were also discussed. It is expected that the obtained polyamides have the potential for biomaterial applications.
469
Authors: Tetsuya Tateishi, Guo Ping Chen
Abstract: A new method for the preparation of biodegradable porous scaffolds has been developed by using preprepared ice particulates as porogen material. A novel kind of hybrid biodegradable porous scaffold has been developed by forming collagen microsponges in the pores or interstices of a synthetic polymer sponge or mesh. A hybrid sponge of synthetic polymer, collagen and hydroxyapatite has been developed for hard tissue engineering. Bovine articular cartilage-like tissue has been engineered by culturing chondrocytes in the PLGA-collagen scaffolds.
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Authors: Jin Iida, Takafumi Yoshikawa, Y. Ueda, Hajime Ohgushi, Toshimasa Uemura, Y. Enomoto, Kunio Ichijima, Yoshinori Takakura, Tetsuya Tateishi
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Authors: Takafumi Yoshikawa, Hajime Ohgushi, Toshimasa Uemura, Y. Ueda, Hideo Nakajima, Y. Enomoto, Kunio Ichijima, Yoshinori Takakura, Tetsuya Tateishi
475
Authors: Tetsushi Taguchi, H. Saito, Masashi Iwasashi, Masataka Sakane, S. Kakinoki, Naoyuki Ochiai, Tetsuya Tateishi
Abstract: A biocompatible glue consisting of human serum albumin (HSA) and citric acid derivative (CAD), named CAD-A glue was developed. CAD was successfully synthesized by the reaction between citric acid and N-hydroxysuccinimide in the presence of 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride. When the CAD-A glue was applied to the collagenbased casings, it took 7 min to obtain half of maximum bonding strength (760 g/cm2). The bonding strength of this glue to collagen-based casings increased with increasing of HSA concentration.The bonding strength of CAD-A glue increased with increasing CAD concentration up to 200 mM, and then decreased with increasing CAD concentration under the fixed HSA concentration (50 w/w%). The CAD-A glue showed excellent wound closure ability rather than fibrin glue when applied to the mouse skin. These results suggested that this developed glue had both tissue compatibility and bonding strength for use in clinical field.
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