Papers by Author: Ulrike Deisinger

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Authors: Ulrike Deisinger, Frauke Stenzel, Günter Ziegler
Authors: Ulrike Deisinger, Sabine Hamisch, Matthias Schumacher, Franzika Uhl, Rainer Detsch, Günter Ziegler
Abstract: In the last few years new fabrication methods, called rapid prototyping (RP) techniques, have been developed for the fabrication of hydroxyapatite scaffolds for bone substitutes or tissue engineering applications. With this generative fabrication technology an individual tailoring of the scaffold characteristics can be realised. In this work two RP techniques, a direct (dispense-plotting) and an indirect one (negative mould technique), are described by means of fabricating hydroxyapatite (HA) scaffolds for bone substitutes or bone tissue engineering. The produced scaffolds were characterised, mainly regarding their pore and strut characteristics. By these data the performance of the two fabrication techniques was compared. Dispense-plotting turned out to be the faster technique while the negative mould method was better suited for the fabrication of exact pore and strut geometries.
Authors: Ulrike Deisinger
Abstract: For tissue regeneration in medicine three-dimensional scaffolds with specific characteristics are required. A very important property is a high, interconnecting porosity to enable tissue ingrowth into the scaffold. Pore size distribution and pore geometry should be adapted to the respective tissue. Additionally, the scaffolds should have a basic stability for handling during implantation, which is provided by ceramic scaffolds. Various methods to produce such ceramic 3D scaffolds exist. In this paper conventional and new fabrication techniques are reviewed. Conventional methods cover the replica of synthetic and natural templates, the use of sacrificial templates and direct foaming. Rapid prototyping techniques are the new methods listed in this work. They include fused deposition modelling, robocasting and dispense-plotting, ink jet printing, stereolithography, 3D-printing, selective laser sintering/melting and a negative mould technique also involving rapid prototyping. The various fabrication methods are described and the characteristics of the resulting scaffolds are pointed out. Finally, the techniques are compared to find out their disadvantages and advantages.
Authors: Ulrike Deisinger, Frauke Stenzel, Günter Ziegler
Authors: Rainer Detsch, Franzika Uhl, Ulrike Deisinger, Günter Ziegler
Abstract: The aim of this study is to analyse the influence of differently fabricated HA-scaffolds on bone marrow stromal cells. Therefore, three methods were used (a polyurethane (PU)-replica technique, the dispense-plotting and a negative mould technique) to produce porous hydroxyapatite (HA) ceramics. The different HA-scaffolds were then cultivated with an osteoblastic precursor cell line. In our study, highest cell proliferation and differentiation was achieved by using (PU)-replica technique. However, this study shows also that all three types of scaffolds are suitable for tissue engineering applications and as bone substitute material. The knowledge about the influence of pore size and geometry on the cell behaviour will help to tailor scaffolds, by different 3D fabrication methods, for the needs of tissue engineering laboratories or patients.
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