Papers by Keyword: Cell Culture

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Authors: Faik N. Oktar, Livia E. Zdrentu, S. Petrescu, Patricia Valério, L.S. Ozyegin, Ismail Peker
Abstract: Hydroxyapatite (HA) material, collected from human tooth, was calcined and then separated very easily to enamel HA (EHA) and dentine HA (DHA). The EHA and DHA were plasma sprayed on pre-prepared titanium implants. HeLa (a lineage malignant fibroblast cell) and primary fibroblast cells cell lines were used in this study. The results using fibroblasts from primary culture are interesting since the cells did not die or became apoptotic. However, this does not mean that the present method reveals the complete biocompatibility of the tested HA material. This study aimed to investigate if fibroblasts with fluorescent tests could be used as a secondary evaluation method for biocompatibity fibroblasts cell culture studies prior to osteoblasts cell culture.
Authors: Larry L. Hench, Julia M. Polak
Abstract: Historically the function of biomaterials has been to replace diseased, damaged and aged tissues. First generation biomaterials, including bio ceramics, were selected to be as inert as possible in order to minimize the thickness of interfacial scar tissue. Bioactive glasses provided an alternative from the 1970’s onward; second generation bioactive bonding of implants with tissues and no interfacial scar tissue. This chapter reviews the discovery that controlled release of biologically active Ca and Si ions from bioactive glasses leads to the up-regulation and activation of seven families of genes in osteoprogenitor cells that give rise to rapid bone regeneration. This finding offers the possibility of creating a new generation of gene activating bioceramics designed specially for tissue engineering and in situ regeneration of tissues.
Authors: Bao Yue Zhang, Hui Xue Song, Tan Chen, Zhan Hui Wang
Abstract: Traditional cell-based assays such as cell immunoassay that utilizes plastic (chamber slides, dishes, microtiter plates), Magnetic bead, enzyme-linked immunsorbent assays (ELISA) [1], FACS cell sorting is labor intensive, time consuming, and requires a large numbers of cells or reagents. In this report, a microfluidic device integrated with cell culture, washing, fixation, and antigen-antibody reaction is presented for high-throughput immunoassay. Using this microfluidic device, each assay can be performed on a small number of cells and nanolitre or picolitre of reagents, this is particularly beneficial for rare or expensive cell types such as stem cells, or flow sorted cell populations. The capability of the microfluidic device was demonstrated for seeding human umbilical cord blood mesenchymal stem cells (UC-MSCs) in chambers and detecting the expression of surface markers (CD34, CD44, CD45, CD73, CD105, HLA-DR) by immunofluorescence assay.
Authors: M. Sato, N. Ando, S. Ozawa, Hiroyuki Miki, K. Hayashi, M. Kitajima
Authors: Artem Minin, Ilya Byzov, Mikhail Uimin, Anatoly Ye. Ermakov, Nina Shchegoleva, Sergey Zhakov, Leonid Smoluk, Maria Ulitko, Artem Minin
Abstract: The simultaneous combination of optical and magnetic properties of nanoparticles would greatly benefit in vivo disease diagnosis as well as in situ monitoring of cell in cell culture. The most promising application of magnetic particles in biomedicine is MRI contrast enhancement and magnetic hyperthermia. Another important thing is the determination of exact localization of nanoparticles in the cell culture that can be defined by e.g. optical way. In our investigation we used the iron nanoparticles encapsulated in carbon as a magnetic component and carbon quantum dots as an optical labels to provide the photostability and fluorescence in a wide range of wavelengths. In order to avoid the fluorescence quenching in bimodal particles the optical and magnetic components should be separated by insulator layer. To create the optimal bimodal nanoparticles for this purpose the non-typical configuration of nanocomposites was realized, namely, a fluorescent core was separated from the coated magnetic particles by silicon dioxide matrix. Finally, it was shown that these bimodal nanocomposites demonstrate the high magnetic properties, good visualized ability and low toxicity for living cells as well.
Authors: Jan Schrooten, Siegfried V.N. Jaecques, R. Eloy, Claire Delubac, Christoph Schultheiss, Patrice Brenner, Lothar H.O. Buth, Jan Van Humbeeck, Jos Vander Sloten
Authors: Faik N. Oktar, Patricia Valério, Gültekin Göller, Simeon Agathopoulos, Alfredo Goes, M. Fatima Leite
Abstract: The in vitro biocompatibility of aragonite material obtained from inner and out layers of four different molluscs was tested. After grinding and sieving, the obtained fine powders were put in contact with primary osteoblasts derived from rat calvariae. The viability of the cells increased at about 10% in the presence of powders derived from Vennus Gallina outer layer and from Pecten Jacobaeus inner layer. In the case of the presence of the other 6 tested powders, there was no statistical difference in cells’ viability. With regard to alkaline phosphatase production, all the tested powders induced a decrease of the production of this enzyme by osteoblasts. There was no evidence of any alterations in collagen production.
Authors: Bo Li, Li Hua Li, Chang Ren Zhou
Abstract: Solid freeform fabrication, known as rapid prototyping (RP) technology allows in designing the scaffold with pre-defined and controlled external and internal architecture.In this study we produce scaffolds with network of chitosan fibrils that mimic the extracellular matrix produced by the cells. These network scaffolds also consisting of nanoparticles of hydroxyapatite (HA) for stabilisation of scaffolds are characterised by environmental scanning electron microscopy and mechanical properties. ESEM showed that the scaffolds possess macropore (300µm), micropore and fibre network structure. The compressive strength and elastic modulus (E) for the scaffolds are 0.54± 0.02 MPa and 6.13± 0.60 MPa, respectively, which are increasing obviously. The biocompatibility of the woodpile-network scaffolds was investigated with osteoblastic cells. The result showed the distribution and proliferation of osteoblast orients along the chtosan fibre network, preferentially. After 4 weeks of culture, macropore channels are covered by cells in large part,while the areas without chitosan fibre network are covered rarely. The properties of these scaffolds indicate that they can be used for bone tissue engineering applications.
Authors: Yan Bo Feng, Wei Qi Yan, Di Sheng Yang, Jie Feng, Xiao Xiang Wang, Sam Zhang
Abstract: The objective of this study was to evaluate the interface shear strength and the responses of osteoblast-like cells to titanium implants with a sandblasted and acid-etched surface modified by alkali and heat treatments (SLA-AH). The implants with machined and SLA surface served as controls. Each type of implant was characterized by scanning electron microscopy (SEM) and energy-dispersive x-ray (EDX) analysis. In vitro assays were made using human osteoblast-like cell culture on different surfaces. The rectangle plates were also transcortically implanted into the proximal metaphysis of New Zealand White rabbit tibiae. After 4, 8 and 12 weeks implantation, mechanical and histological assessments were performed to evaluate biomechanical and biological behavior in vivo. By SEM examination, SLA surface combined with AH treatments revealed a macro-rough surface with finely microporous structure. The in vitro assays showed that the SLA-AH surfaces exhibited more extensive cell deposition and improved cell proliferation as compared with controls. Pull-out test demonstrated that the SLA-AH treated implants had a higher mechanical strength than the controls at all interval time after implantation. Histologically, the test implants revealed a significantly greater percentage of bone-implant contact when compared with controls. The results of this study suggest that a useful approach by combined processes could optimize implant surfaces for bone deposition and produce distinct biological surface features.
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