Papers by Keyword: Alternate Soaking Process

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Authors: Shinobu Tanaka, Masataka Sakane, M. Tanaka, Isamu Yamaguchi, Hitoshi Shimojo, Kenji Kato, Tetsuya Tateishi, Yutaka Miyanaga
Authors: Mitsuru Takemoto, Shunsuke Fujibayashi, J. Suzuki, Tadashi Kokubo, Takashi Nakamura
Abstract: The nano-composite of a ceria-stabilized tetragonal zirconia polycrystals (Ce-TZP) and alumina (Al2O3) polycrystals (Ce-TZP/Al2O3) is attractive as a load-bearing bone substitute because of its mechanical properties and phase stability. We have developed a new method of hydrofluoric acid and heat treatment (HFT) to give a microporous structure to the surface of this Ce-TZP/Al2O3 nanocomposite ceramic. Bone-bonding ability of a microporous surface and calcium phosphate coating on Ce-TZP/Al2O3 composite has been investigated through in vivo detaching model. Thin calcium phosphate coating layer was added by alternate soaking process, and thick CaP layer was produced by soaking in simulated body fluid for 5 days. HFT treated Ce-TZP/Al2O3 composite showed high bone-bonding ability compared with the control group. Thick and thin CaP coating accelerated bone-bonding ability in early post-implantation period. The submicron microporous surface was beneficial for achieving mechanical interlocking between the ceramic and surrounding bone. These results suggest the possibility of using a Ce-TZP/Al2O3 nanocomposite ceramic with microporous surface and calcium phosphate coating as the bearing material for uncemented total joint replacements.
Authors: Shinobu Kobayashi, Masataka Sakane, Hirotaka Mutsuzaki, Hiromi Nakajima, M. Tanaka, Yutaka Miyanaga, Naoyuki Ochiai
Abstract: We hybridized calcium phosphate (CaP) with human semitendinosus and gracilis (ST/G) tendon grafts using an alternate soaking process. To evaluate quantitatively and histologically assess the CaP hybridized human ST/G tendon grafts, we classified them into three groups according to their soaking time – number of soaking cycle: 30 sec – 20 cycles (Group A), 1 min – 15 cycles (Group B), 3 min – 5 cycles (Group C). The tendon grafts were divided into three parts: tibial end (TE), femoral end (FE) and intra-articular (IA) portion. TE was secured using the Krackow technique with No. 2 nonabsorbable sutures, and an Endobutton-CL (Smith & Nephew, USA) was passed through the looped FE, as performed clinically. Then, the IA portion was covered with the sleeve of a rubber glove to prevent CaP hybridization. More soaking cycles induced greater deposition of CaP in the tendon grafts when the total soaking time was the same. Covering the IA portion with a rubber sleeve prevented of CaP deposition. A large amount of CaP in TE was deposited because suture holes increased the total contact area with the solutions.
Authors: Hirotaka Mutsuzaki, Masataka Sakane, Yumi Katayoshi, Sinya Hattori, Hiromi Nakajima, M. Tanaka, Naoyuki Ochiai
Abstract: Calcium phosphate (CaP) hybridized to a whole tendon graft delayed cell repopulation in anterior cruciate ligament (ACL) reconstruction in rabbits. However, a tendon graft masked with an adhesive tape at the intra-articular (IA) portion to prevent CaP hybridization did not delay cell repopulation. Synovial tissues can adhere to the tendon graft and can invade the tendon graft masked at the IA portion. The masking induced an effect similar to that of the unhybridized tendon graft. The CaP hybridized tendon grafts masked at the IA portion showed cell repopulation 2 weeks earlier than the unmasked CaP hybridized tendon grafts.
Authors: Ling Li Li, Guang Li, Jian Ming Jiang, Yue Wen Guo
Abstract: Biomineralization on surface-modified poly (ε-caprolactone) (PCL) through an alternate soaking process was investigated. A poly(vinyl alcohol) (PVA)-coating on PCL substrate prepared by repetitive adsorption/ drying in an aqueous PVA solution could accelerate hydroxyapatite (HA) deposition while PVA-PCL soaked in aqueous solutions containing Ca2+ and PO3-4 ions alternately. Fourier transform infrared (FTIR) and X-ray diffraction of the apatite verified the formed hydroxyapatite in these processes exhibited a close resemblance with calcium phosphates (HA, Ca10(PO4)6(OH)2). It was also found that the amount of HA formed increased with both soaking with both soaking cycles and deposition amount of PVA on PCL. This study suggested that simple PVA coating on PCL substrate could serve as a novel way to accelerated HAP formation via alternating soaking.
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