Papers by Keyword: Bioreactor

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Authors: Helena Marques da Silva, Ingrid Russoni de Lima, Paula Gil Patrício Bezerra, Gabrielly Peregrino, Gloria Dulce de Almeida Soares
Abstract: The knowledge of the degradation process is an important role to understand and predict in vitro results. Granules of strontium-hydroxyapatite (Sr-HA) were submitted to a degradation process under dynamic conditions using a bioreactor system and using a solution with pH=5. The degradation process under dynamic conditions can be represented by a curve in which the calcium content firstly increases (dissolution part) followed by calcium decreasing (reprecipitation). The use of a low pH solution does not affect the curve’s shape but reduces the precipitation step.
Authors: Hui Rong Xu, Shao Chun Sui, Xiao Hong Wang, Yong Nian Yan, Ren Ji Zhang
Abstract: Bioreactor technology is a branched research area of tissue engineering. Dynamic culture environment mimicking in vivo pulsatile conditions could be achieved by bioreactor otherwise might not through static cultures. In this paper, we present a new type of pulse bioreactor which can provide arbitrary and easily adjustable circulatory flow conditions of 0 - 0.2 MPa pressure. The pulse amplitude range was 0 - 7%. The pulse frequency can be adjusted between 0 and 80 times/min. In addition, the new type of pulse bioreactor can be sterilized and dismantled easily. This bioreactor has been used in dynamic culture of assembled adipose derived stem cells (ADSCs) and shown promise in tissue engineering.
Authors: Peng Xu, Yu Tao Men, Bao Shan Xu, Qiang Yang, Jun Lu, Chun Qiu Zhang
Abstract: The mechanical environment has an important influence on biological behavior of the human musculoskeletal system. Either in vivo or in vitro culture, the growth and development of the musculoskeletal tissue depend on the mechanical environment. Now we propose a new loading device which uses a voice coil motor as driving force to further optimize tissue engineering bioreactor. It can not only provide tissue engineering in vitro culture with different sizes and frequency of loading environments within the physiological range, but also detect the mechanical properties of the culture in building process. This device, which uses the voice coil motor as driving force and closed-loop encoder to control displacement, has the characteristics of low power loss, high acceleration and smooth loading, and can achieve a high-precision loading process with different ranges such as from high-speed to low-speed. The device can facilitate the load research in build process of the engineered musculoskeletal system.
Authors: Da Shuai Wang, Yu Tao Men, Li Lan Gao, Xin Dong, Jun Lu, Chun Qiu Zhang
Abstract: A bioreactor has provided a new way for the biomedical tissue engineering, and it is a device system that simulates the metabolism and movement of organisms to obtain target product in vitro. By reference and study of the bioreactors, based on the linear motor of high-precision and high-frequency characteristics, we have designed a bioreactor with varieties of biomechanical functions by using CAD design software, that is especially used in the tissue engineering for mechanical stimulation. The bioreactor can not only load on the cultures under rolling, sliding or their combination as well as tension and compression, but also on the cultures under high-frequency and dual-frequency forces. It will provide a more effective research platform for tissue culture and regeneration.
Authors: Zi Ning Wang, Xing Yu Liu, Qi Yuan Gu, Ming Jiang Zhang
Abstract: Smelting waste water is typically considered as an environmental pollutant. It will contaminate watercourses, crops and aquatic organism when it is discharged into the environment. Traditionally, the metallurgy plant treats this kind of waste water with neutralization method. However, the neutralization method usually ends up with large amounts of neutralization sludge which is a typical kind of hazardous solid waste. In our research, we used an up-flow anaerobic sludge bed to treat smelting waste water which was neutralized to a certain pH. We combined the traditional neutralization method with microbial technique. The neutralized water was pumped into the bioreactor that had been inoculated with sulfite reducing bacteria on a novel expanded hollow glass bead. In our research, we decreased the lime consume and sludge production, and we decrease the metal ion and sulfate in the smelting waste water at the same time. We combined the traditional neutralization method with microbial treatment and developed a novel solution to deal with smelting waste water. After 8 weeks, the concentration of Zn2+ was decreased by 34.7%, and the concentration of SO42- was decreased by 58.3%.
Authors: Christine Poon, Mei Zhang, Andrew Ruys, Angela Hong, Christelle Catuogno, Philip Boughton
Abstract: Tissue engineering of airway tissues poses many complex challenges. As tissue form is determined by function and vice versa, it is necessary to consider mechanical and physiological constraints in conjunction with standard biologic and biochemical factors when culturing tissues in vitro. This study involved the development and validation of a novel 3-dimensional (3-D) construct with the capacity to periodically expose a cell scaffold to air and medium at application of physiologic strain rates. The ultimate objective was to mimic respiratory conditions experienced by airway tissues during breathing whilst ensuring compatibility with proven cell culture techniques. The Biaxx design consists of an elastomeric porous synthetic scaffold integrated with a unique biopolymer coupling unit which engages with an IAXSYS bioreactor actuator. Uniform biaxial strain was imparted by the coupling unit whilst simultaneously creating a periodic air-liquid interface. Biaxx scaffolds with and without a coating of particulate 45S5 bioglass were employed in an assay to assess cell attachment and proliferation whilst subject to periodic strain. Physiologic lung tissue strain of 5-15% was achieved for over 200,000 cycles at 0.2Hz. Preliminary biological studies with H460 human lung carcinoma cells confirmed cell attachment, growth and proliferation on this promising construct.
Authors: Yang Liu, Zhong Ping Qiu, Gui Chen Wang
Abstract: Urbanization, economic growth, and the continuing improvement in living standards have all contributed to the increase of municipal solid waste. More effective technology is needed to improve the conditional landfill of municipal solid waste. Multifunctional microbe community introduced in bioreactor landfill accelerates the degradation of leachate of MSW( Municipal Solid Waste). The results show that introduced microbes shorten the acid formation phase effectively, 208 days earlier than contrast group; introduced microbes accelerated the degradation of leachate. Concentration of COD of leachate decreases significantly and remains 3000 mg/L after 505th day, 187 days earlier than contrast group. Concentration of ammonia nitrogen of leachate decomposes more rapidly (63 days earlier) and finally reaches 0.025 g/L at 700th day that meets the national pollution - control requirements of MSW (GB 16889-2008).
Authors: B. Obradović, A. Osmokrović, B. Bugarski, D. Bugarski, G. Vunjak-Novaković
Abstract: Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 μm) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.
Authors: I. Nettleship
Authors: Pasquale Stano
Abstract: In this article I discuss the backgrounds, some technical insights, and the novel developments of a bioengineering approach to semi-synthetic minimal cells that is currently pursued within the EU project SYNTHCELLS. Originally developed by Pier Luigi Luisi and coworkers at the Swiss Federal Institute of Technology (ETH, Zurich), the project aims to the construction of liposome-based bioreactors, which display living properties, although at a minor complexity level.
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