Papers by Keyword: Blood Compatibility

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Authors: Zhong Hai Yang, Yi Xu, Ping Yang, Yong Xiang Leng, Nan Huang
Abstract: Non-stoichiometric titanium dioxide (TiO2-x) thin films were obtained by unbalanced reactive pulsed magnetron sputtering and subsequently modified by hydrogen plasma. To investigate the influence of hydrogen plasma on the properties of the films, time and temperature were changed during the process with a fixed vacuum pressure of 10 Pa. The film structure, composition, resistance and blood compatibility were studied. The results that the concentration of Ti4+ decreases with the increasing of treated time and substrate temperature, which results in existence of oxygen vacancy and increase of conductivity. It is found that Ti-O film treated in 110°C plasma for 15min represents the lowest thrombosis risks, which could be mainly attributed to its characteristic of n-type semiconductor caused by appropriate oxygen vacancy forming in hydrogen plasma treatment.
Authors: Zhe Wei Huang, Jiao Sun, Li Yi Shi, Da Wei Zhang
Abstract: Nano-sized silicon dioxide (SiO2) is widely utilized in artificial bone, artificial tooth, interventional catheters and drug delivery system,so it is imperative to study SiO2 NPs on the role of the body damage. Consulted with standard reference of GB/T16886, 20 and 80 nm SiO2 NPs were selected to prepare suspension (1mg/ml) for subacute systemic toxicity; 2000mg/ml suspension for MTT; and extracts (0.1g/ml) for hemolytic test. The results show that the hemolysis rate are all more than 5%, after its continuous injection of 2W nanoparticles into SD rats and New Zealand Rabbits, the alterations on the hemoglobin, hematocrit, mean corpuscular volume and other indicators have gained in blood routine test, and there was a marked inhibition on the L929 cells of SiO2 NPs. Therefore, these two kinds of particle size of SiO2 NPs have certain extent of injury effect on the blood system, and the contact time of NPs in the blood compatibility evaluation has play a more important role than the others.
Authors: Zhen Yi Shao, Ping Yang, Yong Xiang Leng, Qi Zhang, Guo Jiang Wan, Hong Sun, Guang Jun Cai, Nan Huang
Abstract: Si-N-O films have drawn researcher’s much attention recently due to their potential superiority in blood compatibility of biomaterials. In this paper, Si-N-O films were synthesized on <100> silicon substrates by pulsed reactive unbalanced magnetron sputtering a single crystal silicon target with high purity in a mixture atmosphere of Ar and N2. XPS and FTIR results showed the Si-N-O films synthesized at higher N2 flux could be described to random bonding model (RBM). In RBM, the Si2p existed in the form of a-Si3N4 and SiNνO4-ν (ν=0,1,2,3,4) components. Platelet adhesion behavior on Si-N-O films was assessed by platelet adhesion test and Lactate dehydrogenase (LDH) assay, qualitatively and quantitatively separately. The correlativity of film chemical structure and blood compatibility was investigated. The results of platelet adhesion and activation showed that the RBM film with higher N/O ratio exhibited favorable blood compatibility. It was shown that the Si-N-O film with specific composition and chemical bonding state was superior in blood compatibility compared to low temperature isotropic carbon (LTIC).
Authors: K. Navaneetha Pandiyaraj, Jan Heeg, Christoph Mewes, Marion Wienecke, Torsten Barfels, V. Uthayakumar, Pi Guey Su
Abstract: Silver containing diamond like carbon films were coated on the surface of polyethylene film (PET) using novel hybrid sputtering method. Polymeric substrates can create soft, flexible, highly absorbent and cost-effective materials by selecting or controlling their molecular structures. The material silver is known to be a potential antibacterial material. The silver containing coating has been potentially recommended for synthesis biomedical materials. In the present work, we discussed the antibacterial activity of the silver containing DLC film coated PET film surfaces which was coated as a function of deposition power level. The surface morphology of the Ag-DLC was analysed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The atomic concentration and structure of the Ag-DLC was measured by energy dispersive X-ray (EDX) and Raman spectroscopy. The hydrophilicity of the films was measured by contact angle analysis. The antibacterial activity of Ag–DLC films were evaluated by bacterial eradication tests with Escherichia coli at incubation time of one day. In addition, blood compatibility of the Ag-DLC films were studied by In Vitro blood compatibility tests. It was found that the surface of the obtained Ag-DLC decreases with increasing the deposition power level. The antibacterial and hemocompatibility of the silver containing DLC film increase gradually with increase of deposition power level. Our results revealed that the Ag-incorporated DLC films are potentially useful as biomedical devices having good antibacterial and hemocompatibility.
Authors: Qiang Liu, Xiao Nong Cheng, Huang Xia Fei
Abstract: TiO2 thin film containing SrFe12O19 powders on the surface of 316L stainless steel and NiTi alloy was prepared through sol-gel method and micro-magnetic field was established by the magnetization process applied on the coating. The coating film was characterized by x-ray diffraction (XRD) and the surface morphology of the coating was observed by scanning electron microscope (SEM). This blood compatibility thin film was evaluated by dynamic cruor time of blood test.The results show that dynamic cruor time of blood is prolonged by the micro-magnetic field of the thin film, indicating improved blood compatibility.
Authors: K. Kurotobi, Akiko Yamamoto, A. Kikuta, Takao Hanawa
Abstract: Microchannel array chips modified by metal deposition or polymer coating were contacted with blood. Titanium (Ti), chromium (Cr) and gold (Au) films were deposited onto the microchannel array chips. Albumin (Alb) and MPC polymer (MPC) were coated onto other chips. Non-treated Si chips were used as a control. Whole blood was collected with 1000 units/ml heparin solution from young healthy volunteers. The passing time of a 100 µl portion of human whole blood through these channels was measured under a pressure difference of 20 cm H2O. Simultaneously, the flow behavior of blood cells in channel was observed by an optical microscope and recorded by a video recorder. Platelet adhesion was observed on Si, Ti, Cr, and especially on Au. The blood pass-through time (BPT) increased in order of Ti, Si, Cr and Au. On the Alb- and MPC-coated chips, platelets were seldom observed and the BPTs were short in comparison with the metal chips. From these consequences, platelet adhesion depended on the materials. The BPT correlated well to the number of adherent platelets on the materials. Therefore, the blood coagulation reaction originated in platelet activation could be detected using microchannel array. We concluded that this method could be applied to evaluate initial blood compatibility of materials within several minutes in vitro.
Authors: Gang Li, Shuang Xia Zhu
Abstract: Owing to its high mechanical strength, chemical inertness, low frictional coefficient, high wear and corrosion resistance properties and so on, carbon nanotubes (CNTs) films have emerged as a potential material for biomedical applications. In order to investigate the blood compatibility properties of CNTs films synthesized on the silicon (Si) substrate using thermal chemical vapor deposition, the blood compatibility was evaluated in vitro by the platelet adhesion and the activated partial thromboplastin time (APTT). The experimental results demonstrated that the Si-CNTs films reduced thrombus formation by minimizing the platelet adhesion, activation , aggregation and had a tendency to retard the intrinsic coagulation activities of blood due to a higher APTT compared to Si, lower temperature isotropic pyrolytic carbon (LTIC) and Polydimethylsiloxane (PDMS) materials. Further, we found that hydrophobic surfaces are more prone to direct cellular motility in comparison with hydrophilic surfaces. It is concluded that CNTs films on the Si substrate were effective for improvement of blood compatibility.
Authors: T. Tao, Gui Cai Li, Y. Yao, X.F. Wang, Ping Yang, J.Y. Cheng, Yong Xiang Leng, Nan Huang
Abstract: Amorphous Ta-O films were synthesized by reactive pulse unbalanced magnetron sputtering system in this paper. Then the well-crystallized Ta-O films were obtained after they were annealed in vacuum at 800°C for 1h. Hydroxyl group on the surface of amorphous tantalum oxide films was prepared by plasma hydrogenation method. The phase structure was investigated by X-ray diffraction (XRD). The hydroxyl group was characterized using Fourier transform infrared spectroscopy (FTIR). The morphology and growth behavior of the vitro platelet adhesion on the as-deposited, annealed and plasma hydrogenated Ta-O films were analyzed through scanning electron microscope (SEM). The results showed that the quantity of platelet adhered onto the annealed surface were less than as-deposited and hydrogenised films. A new method of preparing hydroxyl group without coupling agents on the inorganic biomaterials has been studied by plasma hydrogenation.
Authors: Guo Ming Li, Ya Xin He, Xing Liu, Juan Su, Gao Fei Qu
Abstract: Epoxidized castor oil-based polyurethane were synthesized with epoxidized castor oil (ECO) and isophorone diisocyanate (IPDI) as the main raw materials. Epoxidized castor oil-based polyurethane (ECOPU) films were impregnated in different concentrations of chitosan (CS) solution containing acetic acid to prepare CS/ECOPU composite film. The structure and morphologies of the film have been characterized by means of mass change test, infrared spectroscopy, and optical polarization microscope. The recalcification time and dynamic blood-clotting tests were used to characterize the blood compatibility of CS/ECOPU composition film. The result showed that CS has been grafted onto the surface of ECOPU film. The material had good blood compatibility, and can be used as biomedical materials.
Authors: Li Li, H. Zhao, Wei Wang, F.F. Nie
Abstract: The magnetic Fe3O4 nanoparticles had been synthesized by co-precipitation process and surface treatment by silane coupling agent (KH570). The magnetic Fe3O4/PMMA nanocomposite films were prepared by blend method, and the chemical structure, mechanical properties, surface morphology and the biocompatibility of the nanocomposite films were studied in this work. The magnetic Fe3O4 nanoparticles were well dispersed in the Fe3O4/PMMA nanocomposite films. The strength of the nanocomposite films, as well as the strain, decreased first and then increased with the increasing of the nanoparticles. The hemolytic ratio indicated that the nanocomposite films had a better blood compatibility.
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