Papers by Keyword: Fibroblast

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Authors: Sira Srinives, Worasak Klongthong, Kanin Selamassakul, Navapass Peaunbida, Suchaporn Jiamjitton, Chinnawich Phamornnak, Chotiros Plabplueng, Bovornlak Oonkhanond, Tawatchai Charinpanitkul
Abstract: Graphene is a single-to-few layer carbon sheet, consisting of carbon atoms hybridizing with one another via sp2 configuration, providing outstanding mechanical, electrical, and electrochemical properties that can be utilized in various applications. In this work, we synthesized graphene powder via a modified Hummers method to obtain electrically insulating graphene oxide (GO), and converted the GO to semiconducting reduced graphene oxide (rGO) using L-ascorbic acid as a reducing agent. The rGO was re-dispersed in DI water and cast on prefabricated electrodes to create an rGO film chemiresistive sensor.The rGO sensor was shown to detect changes in buffer pH and cell concentrations of three different cells: human breast cancer cells (MDA-MB-231); non-small-cell lung cancer cells (A549); and fibroblast cells (L929). Sensor performances were determined in terms of "sensitivity", ratio of normalized resistance change upon exposure to a certain analyte concentration, and the analyte concentration. Our studies serve as strong evidence that the rGO-based chemiresistive sensor can be used for a quick and easy test in indicating buffer pH, or quantifying concentrations of a known cell.
Authors: Lin Li, Yi Li, Jia Shen Li, Lei Yao, Arthur F.T. Mak, Frank Ko, Ling Qin
Abstract: Poly-L-Lactide (PLLA) composites were prepared with nano silver particles with the weight ratios of nano silver particles to PLLA (Ag/PLLA) at 0.5%, 2.5%, 5%, 7.5% and 10% (w/w). In vitro cytotoxicity tests were conducted firstly to evaluate the cytotoxicity of the composites. The results indicated that PLLA, 0.5%, 2.5% and 5% Ag/PLLA composites were nontoxic to cells, while 7.5% and 10% Ag/PLLA composites were significantly toxic to cells. Cell proliferation experiments and antibacterial tests were also performed. 5% Ag/PLLA was found the best for cell proliferation with obvious antibacterial property. Thus, Ag/PLLA can be used as nontoxic scaffolds for tissue engineering with antibacterial property.
Authors: Yuli Setiyorini, Sungging Pintowantoro
Abstract: NiTi alloy is one of important materials in orthodontics. Shape memory effect of this material ensures the possibility to deliver highly desireable light forces for thooth movement. Unfortunately, NiTi has problem in the high amount content of Ni that can cause allergy or even risk of poisioning in the human body due to Ni ion released. To overcome this problem, several methods of coatings were performed to prevent the releasing of Ni ion. They were Electropolishing Pretreatment combined with Photo Electrocatalytic Oxidation, TiN Pack Cementite, Advanced Oxidation Process and Biomimetic Hidroxyapatite. The objective of this research was to analyze the effect of various coatings to the biocompatibility of orthodontic wire. Biocompatibility of coated NiTi wire was investigated through MTT assay using BHK-21 fibroblast cell in order to analyze the toxicity of each coating method. In addition, the surface roughness was determined using Atomic Force Microscope (AFM). Furthermore, the attachment and spreading of fibroblast cell were observed by Scanning Electron Microscope (SEM). The result was revealed that biomimetic HA coating has the highest cell viability percentage due to its surface morphology.
Authors: Sandhra Carvalho, Agda Oliveira, Vanessa Andrade, Maria de Fatima Leite, Alfredo Goes, Marivalda Pereira
Abstract: The cementum, a mineralized tissue lining the tooth root surface, is required for development of a functional periodontal ligament. The presence of healthy cementum is considered to be an important criterion for predictable restoration of periodontal tissues lost as a consequence of disease. Despite the importance of cementum to general oral health, very little is known about the cells responsible for the formation of cementum, cementoblasts. The aim of this study was to examine the effect of the ionic products from the dissolution of bioactive glass with 60% of silica ( BG60S ) on the behavior of cementoblasts, osteoblasts and fibroblasts. The cell viability was tested by MTT assay based on mitochondria activity of the cell and Trypan Blue assay based on membrane cell viability. The membrane cell viability measured by Trypan Blue assay showed the beneficial effect on all the cell types tested. It was observed a higher proliferation in the presence of ionic products from dissolution of BG60S when compared to control. In the MTT assay we also observed increased cell viability on all the cell types, but proliferation of cementoblasts was higher (107%) than observed for the other cells (104%) compared to control. The results from this study suggest that Cementoblasts, osteoblasts and fibroblasts are important cells on events that control the development of mineralizing and not mineralizing tissues and the investigation of the comparative behavior of these cells can be a useful experimental model. The observed effect of the bioactive glass particles on cementoblasts shows that this material is an interesting alternative to be used in composite membranes for cementum tissue engineering.
Authors: A. John, H.K. Varma, T.V. Kumari, V.R. Nisha, D. Narayanan
Abstract: Cytotoxicity and cytofunctionality of the in-house prepared novel bioactive glass coated hydroxyapatite (BGHA) is described here as a first step in characterizing the biocompatibility of the material for orthopaedic application. Sintered hydroxyapatite was coated with bioactive glass by a sol-gel method. The BGHA granule was 2 - 4 mm in size with a pore size in the range of 50 – 150 µm. FT-IR spectrum depicted the functional groups and XRD spectrum defined the crystallinity of the material. MTT assay proved high cellular activity and morphologically the cells showed normal proliferation with the expression of actin and vimentin network in the cytoplasm. In the resin sections, cells were found located on the walls of the pores while in osteoblast-cultured granules, cellular matrix was also observed along the pore interconnections. Recrystallization on the surface of the cultured granules did not support the adhesion of cells. The electron diffraction pattern displayed the high crystallinity of the raw granule while a diffused pattern of amorphous apatite like crystallites was observed for the cultured granules. High calcium and low phosphorous concentration was observed in the medium of the cells simultaneously with ALP and osteocalcin production. This pre-clinical evaluation proved BGHA, as a promising material for bone scaffolds.
Authors: Han Ul Lee, Byung Kim, Seung Jae Lee, Geun Bae Lim, Sung Won Kim, Jong Won Rhie, Dong Woo Cho
Abstract: We fabricated micro-patterned 2.5-D scaffolds using micro-end-milling with controlled pore and island sizes and interconnectivity to determine the effects of the micro-patterns on the cell culturing process. Micro-end-milling can easily realize a high aspect ratio and accuracy, and can be applied to various materials, including those that are biocompatible and biodegradable. Sixteen types of micro-patterned scaffolds were designed and fabricated using the micro-end-milling process. Fibroblasts were seeded and cultured to examine the viability of the developed micropatterned scaffolds and their absorbed cell adhesion. The results confirmed that the fabricated micro-patterns functioned successfully as scaffolds.
Authors: Yeon I Woo, Hyun Joo Son, Hye Ryeon Lim, Mi Hee Lee, Hyun Sook Baek, Kazufumi Tsubaki, Jong Chul Park
Abstract: Glucans have been reported to stimulate immunity and to promote wound healing. Adult human dermal fibroblast (aHDF) cultured in serum free (serum-starvation). Proliferation of aHDF was measured at various concentrations of β-glucan by MTT assay, and migration was observed for 36h on microscope. The result of fibroblast bioassay, β-glucan had positive influence. In this study, the direct effects of β-glucan on proliferation and migration of human dermal fibroblasts were examined in vitro. That means β-D-glucan has the effect to enhance proliferation and aHDF migration speed, and has the potential as a wound healing agent.
Authors: Xiu Peng Wang, Atsuo Ito, Xia Li, Yu Sogo, Ayako Oyane
Abstract: FGF-2-apatite and FGF-2-zinc-apatite composite layers were formed on commercially available anodically oxidized Ti external fixation rods using FGF-2-and ZnCl2-containing supersaturated calcium phosphate solutions. The FGF-2-zinc-apatite composite layers precipitated on the Ti external fixation rods significantly enhanced proliferation of fibroblastic NIH3T3 and osteoblastic MC3T3-E1 cells in vitro.
Authors: M.E. Clark, C. Engel, P.J.C. Felstead, D.M. Walter, David Farrar, Gavin S. Walker, Colin A. Scotchford, D.M. Grant
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